PKC gamma Antibody - BSA Free

Immunohistochemistry: PKC gamma Antibody - BSA Free [NBP3-48269] - Immunohistochemistry of paraffin-embedded rat brain tissue slide using (PKC gamma Antibody) at dilution of 1:200

Western Blot: PKC gamma Antibody - BSA Free [NBP3-48269] - Mouse brain tissue were subjected to SDS PAGE followed by western blot with (PKC gamma antibody) at dilution of 1:1000

• Reactivity: Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PKC gamma Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PKC gamma Antibody - BSA Free (NBP3-48269) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: protein kinase C, gamma. (Uniprot# P63318)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRKCG
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:100-1:500
  • Western Blot 1:500-1:2000
• Theoretical MW: 78 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PKC gamma Antibody - BSA Free

• EC 2.7.11
• EC 2.7.11.13
• MGC57564
• PKC gamma
• PKCC
• PKCCSCA14
• PKCG
• PKC-gamma
• PKCGprotein kinase C gamma type
• PRKCG
• protein kinase C, gamma
• SCA14

Background

Protein Kinase C gamma (PKC gamma) is an 80 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol, phosphotidylserine and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC gamma plays a key role in neuronal signal transduction and in regulating intercellular communication. The activation loop threonine (threonine 514 in PKC gamma) of conventional PKCs is phosphorylated by phosphoinositide-dependent kinase-1 (PDK1), which is necessary for its autophosphorylation on threonine 655 in the turn loop, and threonine 674 in the hydrophobic loop of the carboxy terminus, a critical step in generating a catalytically mature enzyme. The phosphorylation of the hydrophobic loop in the carboxyl terminus of PKCs is believed to be a key determinant in regulating PKC interaction with PDK1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKCG