PKC eta Antibody - BSA Free

Immunoprecipitation:PKC eta AntibodyNBP3-30454] -Detection of human PKC-eta by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-PKC-eta antibody NBP3-30454 (lot NBP3-30454-1) used for IP at 6 µg/mg lysate. PKC-eta was also immunoprecipitated by rabbit anti-PKC-eta.. For blotting immunoprecipitated PKC-eta, A304-236A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

• Reactivity: Hu
• Applications: IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PKC eta Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PKC eta Antibody - BSA Free (NBP3-30454) is a polyclonal antibody validated for use in IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Between 300 and 350
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRKCH
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-citrate/phosphate buffer, pH 7 to 8
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for PKC eta Antibody - BSA Free

• EC 2.7.11
• EC 2.7.11.13
• nPKC-eta
• PKC eta
• PKCL
• PKC-L
• PKCLMGC26269
• PKC-LMGC5363
• PRKCH
• PRKCLprotein kinase C eta type
• protein kinase C, eta

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKCH