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PKC delta [p Thr505] Antibody

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Western Blot: PKC delta [p Thr505] Antibody [NBP3-05450] - Western blot analysis of extracts of U-937 cells, using PKC delta antibody (NBP3-05450) at 1:2000 dilution. U-937 cells were treated by PMA/TPA (200nM) for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.
Western Blot: PKC delta [p Thr505] Antibody [NBP3-05450] - Western blot analysis of extracts of U-937 cells, using PKC delta antibody (NBP3-05450) at 1:2000 dilution. U-937 cells were treated by PMA/TPA (200nM) for 30 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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PKC delta [p Thr505] Antibody Summary

Immunogen
A synthetic phosphorylated peptide around T505 of human PKC delta (NP_006245.2).
Modification
p Thr505
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRKCD
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for PKC delta [p Thr505] Antibody

  • EC 2.7.11
  • EC 2.7.11.13
  • MAY1
  • MGC49908
  • nPKC-delta
  • PKC delta
  • PKCD
  • PKCy
  • PRKCD
  • protein kinase C delta type
  • protein kinase C delta VIII
  • protein kinase C, delta

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Flow Cytometry: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - An intracellular stain was performed on HeLa cells with Caspase-3 Antibody (31A1067) - (Pro and Active) NB100-56708 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).
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Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on HeLa cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to Dylight 650.
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PKC delta [p
Thr505] Antibody
NBP3-05450
PKC delta [p Thr505] Antibody
Species: Hu
Applications: WB

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Bioinformatics

Gene Symbol PRKCD