PKC delta Antibodies
We offer PKC delta Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Proximity Ligation Assay, Sandwich ELISA, Western Blot. Each PKC delta Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our PKC delta Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the PKC delta Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our PKC delta polyclonal antibodies and browse our PKC delta monoclonal antibody catalog.
Alternate Names for PKC delta Antibodies
anti-PKC delta antibody, anti-PRKCD antibody, anti-EC 2.7.11 antibody, anti-EC 2.7.11.13 antibody, anti-MAY1 antibody, anti-MGC49908 antibody, anti-nPKC-delta antibody, anti-PKCD antibody, anti-protein kinase C delta type antibody, anti-protein kinase C delta VIII antibody, anti-protein kinase C, delta antibody
15 Results for "pkc-delta" in Primary Antibodies
![Western Blot: PKC delta Antibody (JJ093-02) [NBP2-67912] - Analysis of PKC delta on different lysates using anti-PKC delta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-(JJ093-02)-Western-Blot-NBP2-67912-img0009.jpg "Western Blot: PKC delta Antibody (JJ093-02) [NBP2-67912] - Analysis of PKC delta on different lysates using anti-PKC delta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3")
![Immunocytochemistry/Immunofluorescence: PKC delta Antibody (JJ093-02) [NBP2-67912] - Staining PKC delta in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-(JJ093-02)-Immunocytochemistry-Immunofluorescence-NBP2-67912-img0010.jpg)
![Immunohistochemistry-Paraffin: PKC delta Antibody (JJ093-02) [NBP2-67912] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-PKC delta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-(JJ093-02)-Immunohistochemistry-Paraffin-NBP2-67912-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: PKC delta Antibody (JJ093-02) [NBP2-67912] - Staining PKC delta in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-(JJ093-02)-Immunocytochemistry-Immunofluorescence-NBP2-67912-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PKC delta Antibody (JJ093-02) [NBP2-67912] - Staining PKC delta in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-(JJ093-02)-Immunocytochemistry-Immunofluorescence-NBP2-67912-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PKC delta Antibody (JJ093-02) [NBP2-67912] - Staining PKC delta in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-(JJ093-02)-Immunocytochemistry-Immunofluorescence-NBP2-67912-img0003.jpg)
![Immunohistochemistry-Paraffin: PKC delta Antibody (JJ093-02) [NBP2-67912] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-PKC delta antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-(JJ093-02)-Immunohistochemistry-Paraffin-NBP2-67912-img0004.jpg)
![Immunohistochemistry-Paraffin: PKC delta Antibody (JJ093-02) [NBP2-67912] - Analysis of paraffin-embedded human liver tissue using anti-PKC delta antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-(JJ093-02)-Immunohistochemistry-Paraffin-NBP2-67912-img0005.jpg)
![Immunohistochemistry-Paraffin: PKC delta Antibody (JJ093-02) [NBP2-67912] - Analysis of paraffin-embedded human spleen tissue using anti-PKC delta antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-(JJ093-02)-Immunohistochemistry-Paraffin-NBP2-67912-img0006.jpg)
![Immunohistochemistry-Paraffin: PKC delta Antibody (JJ093-02) [NBP2-67912] - Analysis of paraffin-embedded human tonsil tissue using anti-PKC delta antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-(JJ093-02)-Immunohistochemistry-Paraffin-NBP2-67912-img0007.jpg)
![Immunohistochemistry-Paraffin: PKC delta Antibody (JJ093-02) [NBP2-67912] - Analysis of paraffin-embedded mouse testis tissue using anti-PKC delta antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-(JJ093-02)-Immunohistochemistry-Paraffin-NBP2-67912-img0008.jpg)
![Immunohistochemistry-Paraffin: PKC delta Antibody (JJ093-02) [NBP2-67912] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PKC delta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-(JJ093-02)-Immunohistochemistry-Paraffin-NBP2-67912-img0011.jpg)
![Western Blot: PKC delta Antibody [NBP1-90957] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-Western-Blot-NBP1-90957-img0004.jpg "Western Blot: PKC delta Antibody [NBP1-90957] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4")
![Immunocytochemistry/Immunofluorescence: PKC delta Antibody [NBP1-90957] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90957-img0007.jpg)
![Immunohistochemistry-Paraffin: PKC delta Antibody [NBP1-90957] - Staining of human upper gastrointestinal shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-Immunohistochemistry-Paraffin-NBP1-90957-img0016.jpg)
![Immunohistochemistry-Paraffin: PKC delta Antibody [NBP1-90957] - Staining of human prostate shows high expression.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-Immunohistochemistry-Paraffin-NBP1-90957-img0008.jpg)
![Immunohistochemistry-Paraffin: PKC delta Antibody [NBP1-90957] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-Immunohistochemistry-Paraffin-NBP1-90957-img0009.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PKC delta Antibody [NBP1-90957] - Staining in human prostate and skeletal muscle tissues using anti-PRKCD antibody. Corresponding PRKCD RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-Immunohistochemistry-Paraffin-NBP1-90957-img0010.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PKC delta Antibody [NBP1-90957] - Analysis in human tonsil and skeletal muscle tissues. Corresponding PKC delta RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-Immunohistochemistry-Paraffin-NBP1-90957-img0011.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PKC delta Antibody [NBP1-90957] - Staining of human prostate, skeletal muscle, tonsil and upper gastrointestinal using Anti-PKC delta antibody NBP1-90957 (A) shows similar protein distribution across tissues to independent antibody NBP1-90351 (B).](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-Immunohistochemistry-Paraffin-NBP1-90957-img0012.jpg)
![Immunohistochemistry-Paraffin: PKC delta Antibody [NBP1-90957] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-Immunohistochemistry-Paraffin-NBP1-90957-img0013.jpg)
![Immunohistochemistry-Paraffin: PKC delta Antibody [NBP1-90957] - Staining of human skeletal muscle shows only very weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-Immunohistochemistry-Paraffin-NBP1-90957-img0014.jpg)
![Immunohistochemistry: PKC delta Antibody [NBP1-90957] - Staining of human tonsil shows strong cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-Immunohistochemistry-NBP1-90957-img0015.jpg)
![Western Blot: PKC delta Antibody [NBP1-90351] - Analysis in control (vector only transfected HEK293T lysate) and PRKCD over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-Western-Blot-NBP1-90351-img0011.jpg "Western Blot: PKC delta Antibody [NBP1-90351] - Analysis in control (vector only transfected HEK293T lysate) and PRKCD over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: PKC delta Antibody [NBP1-90351] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90351-img0007.jpg)
![Immunohistochemistry-Paraffin: PKC delta Antibody [NBP1-90351] - Staining of human duodenum shows weak to moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-Immunohistochemistry-Paraffin-NBP1-90351-img0014.jpg)
![Immunohistochemistry-Paraffin: PKC delta Antibody [NBP1-90351] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-Immunohistochemistry-Paraffin-NBP1-90351-img0006.jpg)
![Immunohistochemistry-Paraffin: PKC delta Antibody [NBP1-90351] - Staining of human prostate shows high expression.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-Immunohistochemistry-Paraffin-NBP1-90351-img0008.jpg)
![Immunohistochemistry-Paraffin: PKC delta Antibody [NBP1-90351] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-Immunohistochemistry-Paraffin-NBP1-90351-img0009.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PKC delta Antibody [NBP1-90351] - Staining in human prostate and skeletal muscle tissues using anti-PRKCD antibody. Corresponding PRKCD RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-Immunohistochemistry-Paraffin-NBP1-90351-img0010.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PKC delta Antibody [NBP1-90351] - Analysis in human tonsil and skeletal muscle tissues. Corresponding PKC delta RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-Immunohistochemistry-Paraffin-NBP1-90351-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PKC delta Antibody [NBP1-90351] - Staining of human prostate, skeletal muscle, tonsil and upper gastrointestinal using Anti-PKC delta antibody NBP1-90351 (A) shows similar protein distribution across tissues to independent antibody NBP1-90957 (B).](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-Immunohistochemistry-Paraffin-NBP1-90351-img0013.jpg)
![Western Blot: PKC delta Antibody (7I5U6) [NBP3-16660] - Western blot analysis of extracts of various cell lines, using PKC delta Rabbit mAb (NBP3-16660) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-(7I5U6)-Western-Blot-NBP3-16660-img0004.jpg "Western Blot: PKC delta Antibody (7I5U6) [NBP3-16660] - Western blot analysis of extracts of various cell lines, using PKC delta Rabbit mAb (NBP3-16660) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: PKC delta Antibody (7I5U6) [NBP3-16660] - Immunofluorescence analysis of NIH-3T3 cells using PKC delta Rabbit mAb (NBP3-16660) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-(7I5U6)-Immunocytochemistry-Immunofluorescence-NBP3-16660-img0003.jpg)
![Immunohistochemistry-Paraffin: PKC delta Antibody (7I5U6) [NBP3-16660] - Immunohistochemistry of paraffin-embedded mouse testis using PKC delta Rabbit mAb (NBP3-16660) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-(7I5U6)-Immunohistochemistry-Paraffin-NBP3-16660-img0002.jpg)
![Immunohistochemistry-Paraffin: PKC delta Antibody (7I5U6) [NBP3-16660] - Immunohistochemistry of paraffin-embedded human thyroid cancer using PKC delta Rabbit mAb (NBP3-16660) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-(7I5U6)-Immunohistochemistry-Paraffin-NBP3-16660-img0001.jpg)
![Western Blot: PKC delta Antibody (2E12) [H00005580-M06] - Western Blot analysis of PRKCD expression in A-431 ( Cat # L015V1 ).](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-(2E12)-Western-Blot-H00005580-M06-img0001.jpg "Western Blot: PKC delta Antibody (2E12) [H00005580-M06] - Western Blot analysis of PRKCD expression in A-431 ( Cat # L015V1 ).")
![Immunocytochemistry/Immunofluorescence: PKC delta Antibody (2E12) [H00005580-M06] - Analysis of monoclonal antibody to PRKCD on A-431 cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-(2E12)-Immunocytochemistry-Immunofluorescence-H00005580-M06-img0004.jpg)
![ELISA: PKC delta Antibody (2E12) [H00005580-M06] - Detection limit for recombinant GST tagged PRKCD is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-(2E12)-ELISA-H00005580-M06-img0003.jpg)
![Western Blot: PKC delta [p Ser645] Antibody [NB100-82141] - Western blot analysis of extracts from MCF7 cells using PKC delta (Ab-645) antibody (Line 1 and 2) and PKC delta(phospho-Ser645) antibody (Line 3 and 4).](http://images.novusbio.com/fullsize2/PKC-delta-[p-Ser645]-Antibody-Western-Blot-NB100-82141-img0006.jpg "Western Blot: PKC delta [p Ser645] Antibody [NB100-82141] - Western blot analysis of extracts from MCF7 cells using PKC delta (Ab-645) antibody (Line 1 and 2) and PKC delta(phospho-Ser645) antibody (Line 3 and 4).")
![Immunohistochemistry-Paraffin: PKC delta [p Ser645] Antibody [NB100-82141] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PKC delta (phospho- Ser645) antibody.](http://images.novusbio.com/fullsize2/PKC-delta-[p-Ser645]-Antibody-Immunohistochemistry-Paraffin-NB100-82141-img0005.jpg)
![Western Blot: PKC delta [p Ser645] Antibody [NB100-82141] - Analysis of extracts from PC12 cells untreated or treated with PMA using PKCd(Phospho-Ser645) antibody.](http://images.novusbio.com/fullsize2/PKC-delta-[p-Ser645]-Antibody-Western-Blot-NB100-82141-img0003.jpg)
![Western Blot: PKC delta Antibody (8E12) [H00005580-M07] - Analysis of PRKCD expression in transfected 293T cell line by PRKCD monoclonal antibody (M07), clone 8E12. Lane 1: PRKCD transfected lysatE (77.5 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-(8E12)-Western-Blot-H00005580-M07-img0004.jpg "Western Blot: PKC delta Antibody (8E12) [H00005580-M07] - Analysis of PRKCD expression in transfected 293T cell line by PRKCD monoclonal antibody (M07), clone 8E12. Lane 1: PRKCD transfected lysatE (77.5 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: PKC delta Antibody (8E12) [H00005580-M07] - Analysis of monoclonal antibody to PRKCD on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-(8E12)-Immunocytochemistry-Immunofluorescence-H00005580-M07-img0003.jpg)
![ELISA: PKC delta Antibody (8E12) [H00005580-M07] - Detection limit for recombinant GST tagged PRKCD is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-(8E12)-ELISA-H00005580-M07-img0002.jpg)
![Western Blot: PKC delta Antibody (6A2) [H00005580-M02] - Analysis of PRKCD expression in transfected 293T cell line by PRKCD monoclonal antibody (M02), clone 6A2.Lane 1: PRKCD transfected lysate(77.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-(6A2)-Western-Blot-H00005580-M02-img0006.jpg "Western Blot: PKC delta Antibody (6A2) [H00005580-M02] - Analysis of PRKCD expression in transfected 293T cell line by PRKCD monoclonal antibody (M02), clone 6A2.Lane 1: PRKCD transfected lysate(77.5 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: PKC delta Antibody (6A2) [H00005580-M02] - Analysis of PRKCD over-expressed 293 cell line, cotransfected with PRKCD Validated Chimera RNAi ( Cat # H00005580-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PRKCD monoclonal antibody (M02) clone 6A2 (Cat # H00005580-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-(6A2)-Western-Blot-H00005580-M02-img0004.jpg)
![Sandwich ELISA: PKC delta Antibody (6A2) [H00005580-M02] - Detection limit for recombinant GST tagged PRKCD is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-(6A2)-Sandwich-ELISA-H00005580-M02-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: PKC delta Antibody (8H1) [H00005580-M09] - Analysis of monoclonal antibody to PRKCD on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-(8H1)-Immunocytochemistry-Immunofluorescence-H00005580-M09-img0002.jpg "Immunocytochemistry/Immunofluorescence: PKC delta Antibody (8H1) [H00005580-M09] - Analysis of monoclonal antibody to PRKCD on HeLa cell. Antibody concentration 10 ug/ml")
![Sandwich ELISA: PKC delta Antibody (8H1) [H00005580-M09] - Detection limit for recombinant GST tagged PRKCD is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-(8H1)-Sandwich-ELISA-H00005580-M09-img0003.jpg)
![Western Blot: PKC delta Antibody (K15-K) [NBP1-30125] - Lane 1-100 ng; lane 2-200ng; lane 3-500 ng of human recombinant N-term GST PKC-delta; MW=104kDa.](http://images.novusbio.com/fullsize2/PKC-delta-Antibody-(K15-K)-Western-Blot-NBP1-30125-img0004.jpg "Western Blot: PKC delta Antibody (K15-K) [NBP1-30125] - Lane 1-100 ng; lane 2-200ng; lane 3-500 ng of human recombinant N-term GST PKC-delta; MW=104kDa.")
![Western Blot: PKC delta [p Tyr311] Antibody [NBP3-05431] - Western blot analysis of extracts of U937 and Raw264.7 cells, using PKC delta antibody (NBP3-05431) at 1:1000 dilution. U-937 cells were treated by PMA/TPA (200nM) for 30 minutes. RAW 264.7 cells were treated by PMA (200 nM) for 30 minutes . Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize2/PKC-delta-[p-Tyr311]-Antibody-Western-Blot-NBP3-05431-img0001.jpg "Western Blot: PKC delta [p Tyr311] Antibody [NBP3-05431] - Western blot analysis of extracts of U937 and Raw264.7 cells, using PKC delta antibody (NBP3-05431) at 1:1000 dilution. U-937 cells were treated by PMA/TPA (200nM) for 30 minutes. RAW 264.7 cells were treated by PMA (200 nM) for 30 minutes . Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.")