PKC delta Antibody (K15-K)

Western Blot: PKC delta Antibody (K15-K) [NBP1-30125] - Lane 1-100 ng; lane 2-200ng; lane 3-500 ng of human recombinant N-term GST PKC-delta; MW=104kDa.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IP
• Clone: K15-K
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

PKC delta Antibody (K15-K) Summary

• Description: This antibody is immunoaffinity purified with immunogenic peptide as a ligand.
• Immunogen: Peptide derived from N-terminal sequence of human PKC delta. Antibody recognizes the epitope located between Lys42 - Trp55
• Epitope: Lys42 - Trp55
• Specificity: This antibody is specific for PKC-delta.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PRKCD
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:1000
• Application Notes: Dilutions for IP must be determined by the end user.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Tris/HCl (pH 8.0) and 10 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

Alternate Names for PKC delta Antibody (K15-K)

• EC 2.7.11
• EC 2.7.11.13
• MAY1
• MGC49908
• nPKC-delta
• PKC delta
• PKCD
• PKCy
• PRKCD
• protein kinase C delta type
• protein kinase C delta VIII
• protein kinase C, delta

Background

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (alpha, betaI, betaII and gamma) and novel (n) PKC isoforms (delta, episilon, zeta, eta and theta). Patterns of expression for each PKC isoform differs among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC delta and episilon are independent of Ca2+. On the other hand, nPKC delta and episilon, as well as all of the cPKC members, possess phorbol ester-binding activities and kinase activities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKCD
• Entrez:
  • 5580(Human)
• Uniprot:
  • Q05655()