PKC alpha Antibody (MC5) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: PKC alpha Antibody (MC5) - Azide and BSA Free [NBP2-80911] - PKC alpha (MC5) antibody was tested in SH-SY5Y cells with Dylight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image from the standard format of this antibody.

Immunohistochemistry: PKC alpha Antibody (MC5) - Azide and BSA Free [NBP2-80911] - IHC staining of PKC alpha in mouse brain using DAB with hematoxylin counterstain. Image from the standard format of this antibody.

Flow Cytometry: PKC alpha Antibody (MC5) - Azide and BSA Free [NBP2-80911] - Detection of PKC alpha in Human HeLa Cell Line by Flow Cytometry. Human HeLa cell line was stained with Mouse Anti- PKC alpha Monoclonal Antibody (Catalog # NB600-201, filled histogram), or Mouse IgG2A isotype control (Catalog # MAB003, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a Bio-Techne brand. Image from the standard format of this antibody.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fi, Pm, Pm, Rb, Sh, Ze
• Applications: WB, ELISA, Flow, Func, ICC/IF, IHC, IP
• Clone: MC5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

PKC alpha Antibody (MC5) - Azide and BSA Free Summary

• Immunogen: Purified bovine brain Protein Kinase C
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRKCA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:200-1:500
  • Immunohistochemistry-Frozen 1:200-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Immunoprecipitation 1:10-1:100
  • Radioimmunoassay
  • Western Blot 1:1000
• Application Notes: This PKC alpha Antibody (MC5) is useful for Western blot, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen and paraffin-embedded sections, Immunoprecipitation and Radioimmunoassay. In WB a band can be seen at ~75 kDa representing PKC alpha.

Reactivity Notes

Chicken reactivity reported in scientific literature (PMID: 26719241).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine, 0.15 M NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PKC alpha Antibody (MC5) - Azide and BSA Free

• AAG6
• aging-associated gene 6
• EC 2.7.11
• EC 2.7.11.13
• MGC129901
• PKC alpha
• PKCA
• PKC-A
• PKC-alpha
• PKCAMGC129900
• PRKACA
• PRKCA
• protein kinase C alpha type
• protein kinase C, alpha

Background

Protein Kinase C (PKC) refers to a family of serine/threonine protein kinases grouped by their activation mechanisms. PKC alpha belongs to the conventional PKC group, alongside Beta-I, Beta-II, and Gamma, and is activated by phosphatidylserine in a calcium dependent manner. Furthermore, PKC alpha is localized to the membrane and cytoplasm, but in some cases can translocate to the nucleus. PKC alpha helps to positively and negatively regulate cell proliferation, apoptosis, differentiation, adhesion, and migration via phosphorylation of RAF1, BCL2, CSPG4, TNNT2/CTNT, or MAPK1/3. It also exhibits positive interactions with ADAP1/CENTA1, CSPG4 and PRKCABP. PKC alpha demonstrates anti-apoptotic function in glioma cells and protects them from apoptosis by suppressing the p53/TP53-mediated activation of IGFBP3 (PMCID: PMC1360842). Additional research has shown PKC alpha's involvement in cellular inflammation (PMCID: PMC3080812).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for PKC alpha Antibody (NBP2-80911). (Showing 1 - 1 of 1 FAQ).

1. We are looking into protein kinase C-alpha antibodies for immunohistochemistry on formalin fixed, paraffin embedded tissue. I see that you have many antibodies, including several monoclonals. I wanted to confirm with you that the following antibodies are specific for C-alpha. NB600-201=MC5 (an older paper suggested that this might react with alpha, beta, gamm), NB110-57356=Y124, NB110-57353=Y143. Also, if you have an comparator data or feedback regarding PKC-alpha antibodies for IHC on FFPE, that would be very helpful. We would like to pick 1 antibody that is easy to titer and robust, so that we can move forward with our studies.
   • We have never heard any feedback that these cross-react with other PKC proteins. If you have seen data that MC5 may cross-react, then I would consider avoiding this antibody if you are concerned. The immunogens for NB110-57356 and NB110-57353 come from the N- and C-terminals, which is where the PKC proteins vary the most, so these are not expected to cross-react and we have had no feedback or testing data that suggests that they do. I would recommend NB110-57356, since it has been published with. You may want to review the published data to determine if it seems this antibody will suit your needs.

Bioinformatics

• Gene Symbol: PRKCA