PKC alpha Antibody (MC5) [Janelia Fluor® 549]

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fi, Rb, Sh, Ze
• Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P
• Clone: MC5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 549

PKC alpha Antibody (MC5) [Janelia Fluor® 549] Summary

• Immunogen: Purified bovine brain Protein Kinase C
• Specificity: This antibody is capable of detecting PKC beta as well.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRKCA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Human, mouse, rat, bovine, sheep, rabbit, canine, porcine and fish. Chicken reactivity reported in scientific literature (PMID: 26719241).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for PKC alpha Antibody (MC5) [Janelia Fluor® 549]

• AAG6
• aging-associated gene 6
• EC 2.7.11
• EC 2.7.11.13
• MGC129901
• PKC alpha
• PKCA
• PKC-A
• PKC-alpha
• PKCAMGC129900
• PRKACA
• PRKCA
• protein kinase C alpha type
• protein kinase C, alpha

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for PKC alpha Antibody (NB600-201JF549). (Showing 1 - 1 of 1 FAQ).

1. We are looking into protein kinase C-alpha antibodies for immunohistochemistry on formalin fixed, paraffin embedded tissue. I see that you have many antibodies, including several monoclonals. I wanted to confirm with you that the following antibodies are specific for C-alpha. NB600-201=MC5 (an older paper suggested that this might react with alpha, beta, gamm), NB110-57356=Y124, NB110-57353=Y143. Also, if you have an comparator data or feedback regarding PKC-alpha antibodies for IHC on FFPE, that would be very helpful. We would like to pick 1 antibody that is easy to titer and robust, so that we can move forward with our studies.
   • We have never heard any feedback that these cross-react with other PKC proteins. If you have seen data that MC5 may cross-react, then I would consider avoiding this antibody if you are concerned. The immunogens for NB110-57356 and NB110-57353 come from the N- and C-terminals, which is where the PKC proteins vary the most, so these are not expected to cross-react and we have had no feedback or testing data that suggests that they do. I would recommend NB110-57356, since it has been published with. You may want to review the published data to determine if it seems this antibody will suit your needs.

Bioinformatics

• Gene Symbol: PRKCA