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PKC alpha Antibody (MC5) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Fi, Rb, ShSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
MC5
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 549

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NB600-201AF405
Alexa Fluor 488
NB600-201AF488
Alexa Fluor 647
NB600-201AF647
Alexa Fluor 700
NB600-201AF700
Allophycocyanin
NB600-201APC
Biotin
NB600-201B
DyLight 350
NB600-201UV
DyLight 405
NB600-201V
DyLight 488
NB600-201G
DyLight 550
NB600-201R
DyLight 650
NB600-201C
DyLight 680
NB600-201FR
DyLight 755
NB600-201IR
FITC
NB600-201F
HRP
NB600-201H
Janelia Fluor 646
NB600-201JF646
PE
NB600-201PE
PerCP
NB600-201PCP
Unconjugated
NB600-201
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PKC alpha Antibody (MC5) [Janelia Fluor® 549] Summary

Immunogen
Purified bovine brain Protein Kinase C
Specificity
This antibody is capable of detecting PKC beta as well.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PRKCA
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Human, mouse, rat, bovine, sheep, rabbit, canine, porcine and fish.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for PKC alpha Antibody (MC5) [Janelia Fluor® 549]

  • AAG6
  • aging-associated gene 6
  • EC 2.7.11
  • EC 2.7.11.13
  • MGC129901
  • PKC alpha
  • PKCA
  • PKC-A
  • PKC-alpha
  • PKCAMGC129900
  • PRKACA
  • PRKCA
  • protein kinase C alpha type
  • protein kinase C, alpha

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: PRRT2 Antibody [NBP1-84168] - Immunohistochemical staining of human cerebellum shows strong positivity in neuropil.
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Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.
Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ERK1 mouse mAb with DAB staining.
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
PKC beta Antibody
NBP2-19846
Western Blot: PKC beta Antibody [NBP2-19846] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC beta antibody diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: PKC beta Antibody [NBP2-19846] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: PKC beta Antibody [NBP2-19846] - Paraffin-embedded rat brain. PKC beta antibody diluted at 1:500.
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PKC epsilon
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AF5134
Western blot shows lysates of human, mouse, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PKC epsilon Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5134) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PKC epsilon at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PKC epsilon was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Human/Mouse/Rat PKC epsilon Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5134) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Western blot shows lysates of human brain (hippocampus) tissue and human brain (hypothalamus) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat PKC epsilon Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5134) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PKC epsilon at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Immunocytochemistry/Immunofluorescence: PKC delta Antibody (JJ093-02) [NBP2-67912] - Staining PKC delta in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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TNF-alpha
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210-TA
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
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EphB2 Antibody
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AF467
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Immunocytochemistry/Immunofluorescence: c-Fos Antibody [NB110-75039] - Rat trigeminal using Rabbit antibody to cFos: whole serum at a 1:500 dilution.
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SDS-Page: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260]
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Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - analysis of human breast tumor tissue using an isotype control antibody (top) and p50 antibody (bottom) at 5 ug/ml.
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p38 gamma/SAPK3
Antibody [Unconjugate
AF1347
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Raf-1 Antibody
(563002)
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Raf‑1 was detected in immersion fixed paraffin-embedded sections of human liver array using Mouse Anti-Human/Mouse/Rat Raf‑1 Monoclonal Antibody (Catalog # MAB4540) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(1 Publication)
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Applications: WB, IHC
PKC alpha Antibody
(MC5) [Janelia Fluor& ...
NB600-201JF549
PKC alpha Antibody (MC5) [Janelia Fluor® 549]
Species: Hu, Mu, Rt, Po, Bv, Ca, Fi, Rb, Sh
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for PKC alpha Antibody (NB600-201JF549) (0)

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Product General Protocols

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ICC/IF Video Protocol

FAQs for PKC alpha Antibody (NB600-201JF549). (Showing 1 - 1 of 1 FAQ).

  1. We are looking into protein kinase C-alpha antibodies for immunohistochemistry on formalin fixed, paraffin embedded tissue. I see that you have many antibodies, including several monoclonals. I wanted to confirm with you that the following antibodies are specific for C-alpha. NB600-201=MC5 (an older paper suggested that this might react with alpha, beta, gamm), NB110-57356=Y124, NB110-57353=Y143. Also, if you have an comparator data or feedback regarding PKC-alpha antibodies for IHC on FFPE, that would be very helpful. We would like to pick 1 antibody that is easy to titer and robust, so that we can move forward with our studies.
    • We have never heard any feedback that these cross-react with other PKC proteins. If you have seen data that MC5 may cross-react, then I would consider avoiding this antibody if you are concerned. The immunogens for NB110-57356 and NB110-57353 come from the N- and C-terminals, which is where the PKC proteins vary the most, so these are not expected to cross-react and we have had no feedback or testing data that suggests that they do. I would recommend NB110-57356, since it has been published with. You may want to review the published data to determine if it seems this antibody will suit your needs.

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Blogs on PKC alpha.

Check out the latest blog posts on PKC alpha.
There is nothing beta than PKC Alpha
cAMP-dependent protein kinase (PKA) is a Ser/Thr protein kinase that is highly conserved between species. Three distinct catalytic (C) subunits have been identified, designated C-alpha, C-beta and C-gamma, where C-alpha and C-beta are most closely rel...  Read full blog post.

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Bioinformatics

Gene Symbol PRKCA