PKC alpha Antibody (MC5) [DyLight 405]


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Product Details

Reactivity Hu, Mu, Rt, Po, Bv, Ca, Fi, Rb, ShSpecies Glossary
Applications ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, RIA
Please see the vial label for concentration. If unlisted please contact technical services.

PKC alpha Antibody (MC5) [DyLight 405] Summary

Purified bovine brain Protein Kinase C
This antibody is capable of detecting PKC beta as well.
Protein A purified
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Packaging, Storage & Formulations

Store at 4C in the dark.
DyLight 405
DyLight 405 (A=400, E=420)
50mM Sodium Borate
0.05% Sodium Azide
Protein A purified


  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:200-1:500
  • Immunohistochemistry-Frozen 1:200-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Immunoprecipitation 1:10-1:100
  • Radioimmunoassay
Application Notes
This PKC alpha Antibody (MC5) is useful for Western blot, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen and paraffin-embedded sections, Immunoprecipitation and Radioimmunoassay. In WB a band can be seen at ~75 kDa representing PKC alpha.

Reactivity Notes

Human, mouse, rat, bovine, sheep, rabbit, canine, porcine and fish.

Alternate Names for PKC alpha Antibody (MC5) [DyLight 405]

  • AAG6
  • aging-associated gene 6
  • EC 2.7.11
  • EC
  • MGC129901
  • PKCA
  • PKC-A
  • PKC-alpha
  • PKCAMGC129900
  • protein kinase C alpha type
  • protein kinase C, alpha


Protein Kinase C (PKC) refers to a family of serine/threonine protein kinases grouped by their activation mechanisms. PKC alpha belongs to the conventional PKC group, alongside Beta-I, Beta-II, and Gamma, and is activated by phosphatidylserine in a calcium dependent manner. Furthermore, PKC alpha is localized to the membrane and cytoplasm, but in some cases can translocate to the nucleus. PKC alpha helps to positively and negatively regulate cell proliferation, apoptosis, differentiation, adhesion, and migration via phosphorylation of RAF1, BCL2, CSPG4, TNNT2/CTNT, or MAPK1/3. It also exhibits positive interactions with ADAP1/CENTA1, CSPG4 and PRKCABP. PKC alpha demonstrates anti-apoptotic function in glioma cells and protects them from apoptosis by suppressing the p53/TP53-mediated activation of IGFBP3 (PMCID: PMC1360842). Additional research has shown PKC alpha's involvement in cellular inflammation (PMCID: PMC3080812).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

ICC/IF Video Protocol

FAQs for PKC alpha Antibody (NB600-201V). (Showing 1 - 1 of 1 FAQ).

  1. We are looking into protein kinase C-alpha antibodies for immunohistochemistry on formalin fixed, paraffin embedded tissue. I see that you have many antibodies, including several monoclonals. I wanted to confirm with you that the following antibodies are specific for C-alpha. NB600-201=MC5 (an older paper suggested that this might react with alpha, beta, gamm), NB110-57356=Y124, NB110-57353=Y143. Also, if you have an comparator data or feedback regarding PKC-alpha antibodies for IHC on FFPE, that would be very helpful. We would like to pick 1 antibody that is easy to titer and robust, so that we can move forward with our studies.
    • We have never heard any feedback that these cross-react with other PKC proteins. If you have seen data that MC5 may cross-react, then I would consider avoiding this antibody if you are concerned. The immunogens for NB110-57356 and NB110-57353 come from the N- and C-terminals, which is where the PKC proteins vary the most, so these are not expected to cross-react and we have had no feedback or testing data that suggests that they do. I would recommend NB110-57356, since it has been published with. You may want to review the published data to determine if it seems this antibody will suit your needs.

Secondary Antibodies


Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB600-201APC
DyLight 350 Labeled NB600-201UV
DyLight 405LS Labeled NB600-201V3
DyLight 488 Labeled NB600-201G
DyLight 680 Labeled NB600-201FR
DyLight 755 Labeled NB600-201IR
FITC Labeled NB600-201F
PE Labeled NB600-201PE
PerCP Labeled NB600-201PCP

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Blogs on PKC alpha.

There is nothing beta than PKC Alpha
cAMP-dependent protein kinase (PKA) is a Ser/Thr protein kinase that is highly conserved between species. Three distinct catalytic (C) subunits have been identified, designated C-alpha, C-beta and C-gamma, where C-alpha and C-beta are most closely rel...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol PRKCA

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