PKA C beta Antibody - BSA Free

Western Blot: PKA C beta Antibody - BSA Free [NBP3-46894] - HT-1080 cells were subjected to SDS PAGE followed by western blot with (PKA C beta antibody) at dilution of 1:1000

Immunohistochemistry: PKA C beta Antibody - BSA Free [NBP3-46894] - Immunohistochemistry of paraffin-embedded human medulloblastoma using (PKA C beta antibody) at dilution of 1:50

Immunoprecipitation: PKA C beta Antibody - BSA Free [NBP3-46894] - IP Result of anti-PKA C beta (IP:, 4ug; Detection: 1:500) with mouse brain tissue lysate 2640ug.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PKA C beta Antibody - BSA Free Summary

• Immunogen: protein kinase, cAMP-dependent, catalytic, beta. (Uniprot# P22694)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRKACB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:20-1:200
  • Immunohistochemistry 1:20-1:200
  • Immunoprecipitation 1:200-1:1000
  • Western Blot 1:500-1:2000
• Application Notes: Theoretical Molecular Weight is 46-55 kDa

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PKA C beta Antibody - BSA Free

• cAMP-dependent protein kinase catalytic beta subunit isoform 4ab
• cAMP-dependent protein kinase catalytic subunit beta
• cAPKbeta
• DKFZp781I2452
• EC 2.7.11
• EC 2.7.11.11
• MGC41879
• MGC9320
• PKA C beta
• PKA C-beta
• PKACb
• PRKACB
• protein kinase A catalytic subunit beta
• protein kinase, cAMP-dependent, catalytic, beta

Background

The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Calpha, Cbeta and Cgamma, that each represent specific gene products. Calpha and Cbeta are closely related (93% amino acid sequence similarity), whereas Cgamma displays 83% and 79% similarity to Calpha and Cbeta, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKACB