PKA C alpha Antibody (6D2.1) - BSA Free

Dot Blot: PKA C alpha Antibody (6D2.1) [NBP2-62195] - analysis of GST and GST-fusion proteins using anti-PKAc (6D2.1) and anti-GST (S-tag-05; cat. no. NBP1-48359).The total amount of material spotted on the nitrocellulose membrane is 5 ng/spot.Lane 1: GST-Akt1Lane 2: GST-Akt2Lane 3: GST-Akt3Lane 4: GST-PKAc alphaLane 5: GST-PKAc betaLane 6: GST-PKAc gammaLane 7: GST-MEK 1Lane 8: GST

• Reactivity: Hu
• Applications: WB
• Clone: 6D2.1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

PKA C alpha Antibody (6D2.1) - BSA Free Summary

• Description: Novus Biologicals Mouse PKA C alpha Antibody (6D2.1) - BSA Free (NBP2-62195) is a monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide corresponding to amino acids ESPAQNTAHLDQFERIK of human proteinkinase A c alpha (PKAc alpha).
• Specificity: The antibody C-50 reacts with Cytokeratin peptides 5 and 18 (58 and 45 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRKACA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/ml
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Purity: Protein A purified

Alternate Names for PKA C alpha Antibody (6D2.1) - BSA Free

• cAMP-dependent protein kinase catalytic subunit alpha
• Cs
• EC 2.7.11
• EC 2.7.11.11
• MGC102831
• MGC48865
• PKA C alpha
• PKA C-alpha
• Pkaca
• PKCA1
• PKCD
• PRKACA
• protein kinase A catalytic subunit
• protein kinase, cAMP-dependent, catalytic, alpha

Background

PKA (Protein Kinase A), also known as cAMP-dependent protein kinase, phosphorylates serine or threonine residues in target protein in response to elevated levels of cAMP. Inactive PKA exists as a tetrameric protein composed of two regulatory (R) subunits and two catalytic (C) subunits. Activation occurs when two cAMP molecules bind to each R subunit which causes a conformational change that releases the active C subunits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKACA