PJA2/Praja2 Antibody (1G11) - Azide and BSA Free

Western Blot: PJA2/Praja2 Antibody (1G11) [H00009867-M01] - Analysis of PJA2 expression in transfected 293T cell line by PJA2 monoclonal antibody (M01), clone 1G11.Lane 1: PJA2 transfected lysate (Predicted MW: 78.2 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: PJA2/Praja2 Antibody (1G11) [H00009867-M01] - Detection limit for recombinant GST tagged PJA2 is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 1G11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PJA2/Praja2 Antibody (1G11) - Azide and BSA Free Summary

• Immunogen: PJA2 (NP_055634, 302 a.a. ~ 400 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. REKNHGSSPEQVVRPKVRKLISSSQVDQETGFNRHEAKQRSVQRWREALEVEESGSDDLLIKCEEYDGEHDCMFLDPPYSRVITQRETENNQMTSESGA
• Localization: Cytoplasmic. Endoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus membrane; Peripheral membrane protein. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Note=Localizes at the cytoplasmic side of endoplasmi
• Specificity: PJA2 - praja 2, RING-H2 motif containing
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PJA2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PJA2/Praja2 Antibody (1G11) - Azide and BSA Free

• E3 ubiquitin-protein ligase Praja-2
• EC 6.3.2
• EC 6.3.2.-
• KIAA0438praja2
• Neurodap1
• praja ring finger 2
• Praja2
• RING finger protein 131praja 2, RING-H2 motif containing
• RNF131

Background

PJA2 (Praja 2, RING-H2 motif containing) is able to bind the ubiquitin-conjugating enzyme UbcH5B and has E2-dependent E3 ubiquitin-protein ligase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PJA2
• Entrez:
  • 9867(Human)
• Uniprot:
  • NP_055634(Human)