PITX1 Antibody - BSA Free

Western Blot: PITX1 Antibody [NB100-1546] - Detection of Human PITX by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PITX antibody NB100-1546 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

PITX1 Antibody [NB100-1546] - Detection of human PITX by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PITX antibody NB100-1546 (lot NB100-1546-2) used for IP at 6 ug per reaction. PITX was also immunoprecipitated by a previous lot of this antibody NB100-1546-1. For blotting immunoprecipitated PITX, A300-577A was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PITX1 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 50 and 100 of human Paired-Like Homeodomain Transcription Factor 1 using the numbering given in entry NP_002644.3 (GeneID 5307).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PITX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1-4 ug/mg of lysate
  • Western Blot 1:2500-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PITX1 Antibody - BSA Free

• BFTpituitary homeobox 1
• CCF
• Hindlimb-expressed homeobox protein backfoot
• Homeobox protein PITX1
• paired-like homeodomain 1
• pituitary otx-related factor
• POTXpituitary homeo box 1
• PTX1Paired-like homeodomain transcription factor 1hindlimb expressed homeobox protein backfoot

Background

PITX1 is a homeobox-containing transcription factor involved in the development of many organs. PITX1 has been demonstrated to play a critical role in pituitary organogenesis and can stimulate transcription from most pituitary hormone promoters. PITX1 has also been shown to stimulate the leutinizing hormone (LH)-beta gene promoter and may serve an important role in reproductive gene expression. Alternate names for PITX1 include pituitary homeobox 1, hindlimb-expressed homeobox protein backfoot, BFT, PTX1, and POTX.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PITX1 Antibody (NB100-1546)(1)

Publication using NB100-1546

• Stender JD, Stossi F, Funk CC et al. The estrogen-regulated transcription factor PITX1 coordinates gene-specific regulation by estrogen receptor-alpha in breast cancer cells. Mol Endocrinol 2011-10-01 [PMID: 21868451]

Bioinformatics

• Gene Symbol: PITX1
• Entrez:
  • 5307(Human)
• Uniprot:
  • P78337(Human)