PIP5K2B Antibody - BSA Free

Western Blot: PIP5K2B Antibody [NBP2-19837] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PIP4K2B antibody [N1C1] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: PIP5K2B Antibody [NBP2-19837] - Mouse muscle. PIP4K2B antibody [N1C1] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: PIP5K2B Antibody [NBP2-19837] - MCF7 xenograft, using PIPK I beta antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

• Reactivity: Hu, Mu, Rt, Bv, Xp
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PIP5K2B Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human PIP5K2B. The exact sequence is proprietary.
• Predicted Species: Rat (100%), Xenopus (92%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PIP4K2B
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (92%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris-Glycine (pH7), 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for PIP5K2B Antibody - BSA Free

• 1-phosphatidylinositol-4-phosphate kinase
• 1-phosphatidylinositol-5-phosphate 4-kinase 2-beta
• Diphosphoinositide kinase 2-beta
• EC 2.7.1
• EC 2.7.1.149
• phosphatidylinositol-4-phosphate 5-kinase, type II, beta
• Phosphatidylinositol-5-phosphate 4-kinase type II beta
• phosphatidylinositol-5-phosphate 4-kinase type-2 beta
• phosphatidylinositol-5-phosphate 4-kinase, type II, beta
• PI(5)P 4-kinase type II beta
• PI5P4KB
• PIP4KII-beta
• PIP5K2B
• PIP5KIIB
• PIP5KIIbeta
• PTDINS(4)P-5-kinase
• PtdIns(5)P-4-kinase isoform 2-beta

Background

The protein encoded by this gene catalyzes the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family. The encoded protein sequence does not show similarity to other kinases, but the protein does exhibit kinase activity. Additionally, the encoded protein interacts with p55 TNF receptor. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PIP4K2B
• Uniprot:
  • P78356(Human)