Tubby Antibody

Images

 
Western Blot: Tubby Antibody [NBP2-20758] - Sample (30 ug of whole cell lysate) A: NT2D1 B: PC-3 C: U87-MG D: SK-N-SH 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Tubby Antibody [NBP2-20758] - SKNSH cells.
Immunohistochemistry-Paraffin: Tubby Antibody [NBP2-20758] - Paraffin-embedded mouse fore brain. Tub antibody dilution: 1:500
Western Blot: Tubby Antibody [NBP2-20758] - Sample (30 ug of whole cell lysate) A: Neuro2A B: GL261 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: Tubby Antibody [NBP2-20758] - Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Tubby Antibody [NBP2-20758] - Paraffin-embedded rat fore brain. Tub antibody dilution: 1:500.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Tubby Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Tub. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TUB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine (85%),

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Tubby Antibody

  • rd5
  • tubby (mouse) homolog
  • tubby homolog (mouse)
  • tubby homologue
  • tubby protein homolog

Background

This gene encodes a member of the Tubby family of bipartite transcription factors. The encoded protein may play a role in obesity and sensorineural degradation. The crystal structure has been determined for a similar protein in mouse, and it functions as a membrane-bound transcription regulator that translocates to the nucleus in response to phosphoinositide hydrolysis. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Tubby Antibody (NBP2-20758) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Tubby Antibody (NBP2-20758)

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Blogs on Tubby.

Auditory Infographic: Can you hear me now?
The auditory process involves several structures of the ear to convert sound waves into information that is processed by our brain. Learn more about the auditory process in our infographic below.Novus Biologicals offers reagents mentioned in the i...  Read full blog post.

Can Tubby Make You Tubby?
The TUB gene, which encodes for the protein Tubby, is evolutionarily conserved in human, chimpanzee, dog, cow, mouse, chicken, zebrafish, fruit fly, mosquito, C. elegans, and rice. The gene derives its name from its role in metabolism; mice with a m...  Read full blog post.

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Bioinformatics

Gene Symbol TUB