PIP5K2B Antibody - Azide and BSA Free

Western Blot: PIP5K2B Antibody [NBP1-44261] - Lane 1: Mouse Brain homogenate samples. Load: 10 ug per lane. Primary antibody: PIP5K2B antibody at 0.2ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 54 kDa for PIP5K2B. Other band(s): None.

• Reactivity: Hu, Mu, Rt, Ch, Pm
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PIP5K2B Antibody - Azide and BSA Free Summary

• Description: This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: PIP5K2B Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of human PIP5K2B. (Uniprot: Q8TCG2)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PIP4K2B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000-1:60000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:100-1:500
  • Western Blot 0.2 ug/ml
• Application Notes: This product is tested for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~54 kDa corresponding to the appropriate cell lysate or extract.

Reactivity Notes

Cross-reactivity with PIP5K2B from other sources has not been determined

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 50% (v/v) Glycerol
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Alternate Names for PIP5K2B Antibody - Azide and BSA Free

• 1-phosphatidylinositol-4-phosphate kinase
• 1-phosphatidylinositol-5-phosphate 4-kinase 2-beta
• Diphosphoinositide kinase 2-beta
• EC 2.7.1
• EC 2.7.1.149
• phosphatidylinositol-4-phosphate 5-kinase, type II, beta
• Phosphatidylinositol-5-phosphate 4-kinase type II beta
• phosphatidylinositol-5-phosphate 4-kinase type-2 beta
• phosphatidylinositol-5-phosphate 4-kinase, type II, beta
• PI(5)P 4-kinase type II beta
• PI5P4KB
• PIP4KII-beta
• PIP5K2B
• PIP5KIIB
• PIP5KIIbeta
• PTDINS(4)P-5-kinase
• PtdIns(5)P-4-kinase isoform 2-beta

Background

PIP5K2B - phosphatidylinositol-4-phosphate 5-kinase, type II, beta.Functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PIP5K2B Antibody (NBP1-44261)(2)

Publications using NBP1-44261

• Wei YJ, Sun HQ, Yamamoto M, Wlodarski P, Kunii K, Martinez M, Barylko B, Albanesi JP, Yin HL. Type II phosphatidylinositol 4-kinase beta is a cytosolic and peripheral membrane protein that is recruited to the plasma membrane and activated by Rac-GTP. J Biol Chem;277(48):46586-93. 2002-11-29 [PMID: 12324459]
• Balla,A, Tuymetova,G, Barshishat,M, Geiszt,M Balla,T. Characterization of type II phosphatidylinositol 4-kinase isoforms reveals association of the enzymes with endosomal vesicular compartments, J. Biol Chem 277 (22), 20041-20050. 2002-01-01 [PMID: 11923287]

Bioinformatics

• Gene Symbol: PIP4K2B
• Entrez:
  • 8396(Human)
• Uniprot:
  • Q8TCG2(Human)