LIMS2 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: LIMS2 Antibody [NBP2-31713] - Staining of human cell line BJ shows localization to focal adhesion sites.

Immunohistochemistry-Paraffin: LIMS2 Antibody [NBP2-31713] - Staining of human skin shows low positivity in squamous epithelial cells as expected.

Immunohistochemistry-Paraffin: LIMS2 Antibody [NBP2-31713] - Analysis in human prostate and pancreas tissues. Corresponding LIMS2 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: LIMS2 Antibody [NBP2-31713] - Staining of human kidney shows strong membranous positivity in cells in glomeruli.

Immunohistochemistry-Paraffin: LIMS2 Antibody [NBP2-31713] - Staining of human pancreas shows low positivity in exocrine glandular cells as expected.

Immunohistochemistry-Paraffin: LIMS2 Antibody [NBP2-31713] - Staining of human prostate shows moderate membranous positivity in smooth muscle cells.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

LIMS2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit LIMS2 Antibody - BSA Free (NBP2-31713) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: KRLKKLSELTSRKAQPKATDLNSA
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LIMS2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  LIMS2 Protein (NBP2-31713PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for LIMS2 Antibody - BSA Free

• FLJ10044
• ILK-binding protein
• LIM and senescent cell antigen-like domains 2
• LIM and senescent cell antigen-like-containing domain protein 2
• LIM-like protein 2
• Particularly interesting new Cys-His protein 2
• PINCH2
• PINCH-2

Background

LIMS2 is a focal adhesion protein that associates with integrin-linked kinase (ILK; MIM 602366), a multidomain protein that mediates multiple protein-protein interactions at adhesion sites between cells and the extracellular matrix (ECM).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LIMS2
• Uniprot:
  • Q7Z4I7(Human)