LIMS2 Antibody

Images

 
Western Blot: LIMS2 Antibody [NBP2-31713] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane ...read more
Immunocytochemistry/ Immunofluorescence: LIMS2 Antibody [NBP2-31713] - Staining of human cell line BJ shows localization to focal adhesion sites.
Immunohistochemistry-Paraffin: LIMS2 Antibody [NBP2-31713] - Staining of human cerebral cortex shows strong cytoplasmic positivity in endothelial cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

LIMS2 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: KRLKKLSELTSRKAQPKATDLNSA
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LIMS2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:250
  • Immunocytochemistry/Immunofluorescence 1-4 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
LIMS2 Protein (NBP2-31713PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for LIMS2 Antibody

  • FLJ10044
  • ILK-binding protein
  • LIM and senescent cell antigen-like domains 2
  • LIM and senescent cell antigen-like-containing domain protein 2
  • LIM-like protein 2
  • Particularly interesting new Cys-His protein 2
  • PINCH2
  • PINCH-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LIMS2 Antibody (NBP2-31713) (0)

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Secondary Antibodies

 

Isotype Controls

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Diseases for LIMS2 Antibody (NBP2-31713)

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Bioinformatics

Gene Symbol LIMS2
Uniprot