PIM2 Antibody (447023)

Western blot shows Recombinant Human Active PIM1 (Catalog # 4588-KS), recombinant human PIM2, and recombinant human PIM3 (5 ng/lane). PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/ Mouse/Rat PIM2 Monoclonal Antibody (Catalog # MAB4355) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for PIM2 was detected at approximately 38-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, Raji human Burkitt's lymphoma cell line, SW480 human colorectal adenocarcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and rat brain tissue. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse/Rat PIM2 Monoclonal Antibody (Catalog # MAB4355) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for PIM2 was detected at approximately 38 kDa and 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 447023
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

PIM2 Antibody (447023) Summary

• Immunogen: E. coli-derived recombinant human PIM2
  Met1-Pro311
  Accession # Q9P1W9
• Specificity: Detects human, mouse, and rat PIM2 in Western blots. In Western blots, no cross-reactivity with recombinant human PIM1 or PIM3 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PIM2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PIM2 Antibody (447023)

• EC 2.7.11.1
• pim-2 oncogene
• PIM2
• pim-2h
• proto-oncogene Pim-2 (serine threonine kinase)
• serine/threonine protein kinase pim-2
• serine/threonine-protein kinase pim-2

Background

The three members of the Pim family of proto-oncogenes encode the serine/threonine kinases PIM1, PIM2, and PIM3. Multiple isoforms of PIM2 are expressed in many tissues, with high levels in spleen, thymus, testis, small intestine, and colon. PIM2 is implicated as a mediator of IL-3 survival signaling in hematopoietic cells, and dysregulated PIM2 expression is observed in prostate cancer, lymphoma, leukemia, and multiple myeloma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PIM2 Antibody (MAB4355)(2)

Publications using MAB4355

• D Marino, M Pizzi, I Kotova, R Schmidt, C Schröder, V Guzzardo, I Talli, E Peroni, S Finotto, G Scapinello, AP Dei Tos, F Piazza, L Trentin, V Zagonel, E Piovan High ETV6 Levels Support Aggressive B Lymphoma Cell Survival and Predict Poor Outcome in Diffuse Large B-Cell Lymphoma Patients Cancers, 2022-01-11;14(2):. 2022-01-11 [PMID: 35053500] (Western Blot, Human)
• W Du, T Chen, Y Ni, X Hou, Y Yu, Q Zhou, F Wu, W Tang, G Shi Role of PIM2 in allergic asthma Mol Med Rep, 2017-09-18;0(0):. 2017-09-18 [PMID: 28944837] (ICC, Human)

Bioinformatics

• Gene Symbol: PIM2
• Uniprot:
  • Q9P1W9()