PILR-alpha Antibody [Phycoerythrin]

Human peripheral blood granulocytes were stained with Sheep Anti-Human PILR-a PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6484P, filled histogram) or isotype control antibody (Catalog # IC016P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Phycoerythrin

PILR-alpha Antibody [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human PILR-alpha
  Gln20-Thr196
  Accession # Q9UKJ1
• Specificity: Detects human PILR-alpha in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: PILRA
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8degrees C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PILR-alpha Antibody [Phycoerythrin]

• Cell surface receptor FDF03
• FDF03
• inhibitory receptor PILRalpha
• Inhibitory receptor PILR-alpha
• paired immunoglobin-like receptor alpha
• paired immunoglobin-like type 2 receptor alpha
• paired immunoglobulin-like receptor alpha
• paired immunoglobulin-like type 2 receptor alpha
• PILRA
• PILRalpha
• PILR-alpha

Background

PILR-alpha (Paired Immunoglobulin-like, type 2 Receptor alpha) is a monomeric 44-50 kDa type I transmembrane (TM) paired receptor glycoprotein that belongs to the Ig Superfamily. It is expressed by neutrophils, monocytes, macrophages, CD14 + CD1a - DC, corneal epithelium and retinal pigment cells. It is known to bind to CD99, PANP, Collectin-12, and NPDC-1. The target for PILR-alpha appears to be sialylated O-glycans. PILR-alpha also acts as a receptor for HSV and serves as a negative immunomodulator that contains an ITIM. Mature human PILR-alpha is 284 amino acids (aa) in length. It contains one V-type Ig-like domain in its extracellular region (aa 32-150), and two ITIMs in its cytoplasmic domain (aa 267-272 and 296-301). There are multiple potential splice variants. One is TM and possesses a 35 aa substitution for aa 264-303, while others are soluble, and may either show a deletion of aa 152-224 that may be coupled to the 35 aa substitution noted above, or simply exhibit a 24 aa substitution for aa 152-303. Over aa 20-196, human PILR-alpha shares only 42% aa identity with mouse PILR-alpha , and 89% aa identity with human PILR-beta .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PILRA
• Uniprot:
  • Q9UKJ1(Human)