PILR-alpha Antibody [Unconjugated]

J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse PILR‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4318, filled histogram) or control antibody (Catalog # AB‑108‑C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

• Reactivity: Mu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

PILR-alpha Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse PILR-alpha isoform 1 (R&D Systems, Catalog # 4318-PR)
  Leu21-Val197
  Accession # Q2YFS3
• Specificity: Detects mouse PILR-alpha in direct ELISAs and Western blots. In Western blots, approximately 20% cross-reactivity with recombinant mouse PILR-beta is observed and 5% cross-reactivity with recombinant human PILR-alpha is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PILRA
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PILR-alpha Antibody [Unconjugated]

• Cell surface receptor FDF03
• FDF03
• inhibitory receptor PILRalpha
• Inhibitory receptor PILR-alpha
• paired immunoglobin-like receptor alpha
• paired immunoglobin-like type 2 receptor alpha
• paired immunoglobulin-like receptor alpha
• paired immunoglobulin-like type 2 receptor alpha
• PILRA
• PILRalpha
• PILR-alpha

Background

PILR-alpha (paired immunoglobulin-like type 2 receptor-alpha; also namedFDF03) is one of two members of a small family of immunoregulatory Ig-superfamily receptors (1, 2). It is a counterpart to PILR-beta and it likely gave rise to PILR-beta through gene duplication and rearrangement (1). The PILRs represent one of many pairs of Ig-like domain-containing receptors that participate in immune regulation. PILR-alpha and -beta should not be confused with the similarly named PIRs (also paired immunoglobulin‑like receptors), or the functionally-related SIRP and ILT/LILR/CD85/LIR family of receptors (2). While PIRs, ILTs and SIRPs contain three to six Ig‑like domains in their extracellular region, PILR-alpha and -beta show only one Ig-like region in their extracellular domain (ECD) (1, 2). Mouse PILR-alpha is a monomeric, 271 amino acid (aa) type I transmembrane (TM) protein (3). It contains a 167 aa ECD, a 21 aa TM segment, and a long, 83 aa cytoplasmic region. The ECD shows one V‑type Ig-like domain between aa 39 - 157, while the cytoplasmic region contains two ITIMs (immunoreceptor Tyr-based inhibitory motifs) between aa 265-270 and 294‑299. Given that ITIMs are known to interact with phosphatases such as PTPN6 and PTPN11, the presence of these motifs makes mouse PILR-alpha an inhibitory receptor. In human, activation of PILR-alpha inhibits CD32/Fc gamma RII-induced calcium mobilization (3). Although CD99 is a known ligand for both PILR-alpha and -beta (4), highest affinity binding seems to occur between CD99 and PILR-alpha (4). Mouse PILR-alpha is found on neutrophils and macrophages (4). Mouse PILR-alpha ECD is 43% and 69% aa identical to human and rat PILR-alpha ECD, respectively; it is 75% aa identical to the ECD of mouse PILR-beta (3). One potential isoform of PILR-alpha has been reported. It varies only within the first 28 aa of the signal sequence (5).

1. Wilson, M.D. et al. (2006) Physiol. Genomics 27:201.
2. Lanier, L.L. (2001) Curr. Opin. Immunol. 13:326.
3. Fournier, N. et al. (2000) J. Immunol. 165:1197.
4. Shiratori, I. et al. (2004) J. Exp. Med. 199:525.
5. SwissProt # Q2YFS3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PILR-alpha Antibody (AF4318)(1)

Publication using AF4318

• D Goswami, S März, YT Li, A Artz, K Schäfer, R Seelige, M Pacheco-Bl, D Jing, MG Bixel, M Araki, K Araki, K Yamamura, D Vestweber Endothelial CD99 supports arrest of mouse neutrophils in venules and binds to neutrophil PILRs Blood, 2017-02-21;0(0):. 2017-02-21 [PMID: 28223280]

Bioinformatics

• Gene Symbol: PILRA
• Uniprot:
  • Q2YFS3()