PIK3R4 Antibody (576514) [CoraFluor™ 1]

• Reactivity: Hu
• Applications: WB
• Clone: 576514
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

PIK3R4 Antibody (576514) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: E. coli-derived recombinant human PIK3R4
  Gln58-Arg230
  Accession # Q99570
• Specificity: Detects human PIK3R4 in Western blots.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PIK3R4
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for PIK3R4 Antibody (576514) [CoraFluor™ 1]

• EC 2.7.11.1
• MGC102700
• p150
• phosphatidylinositol 3-kinase-associated p150
• Phosphoinositide 3-kinase adaptor protein
• phosphoinositide 3-kinase regulatory subunit 4
• phosphoinositide-3-kinase, regulatory subunit 4
• phosphoinositide-3-kinase, regulatory subunit 4, p150
• PI3-kinase p150 subunit
• PI3-kinase regulatory subunit 4
• PIK3R4
• VPS 15
• VPS15

Background

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PIK3R4