PIK3R4 Antibody (1G12)

Images

 
Western Blot: PIK3R4 Antibody (1G12) [H00030849-M03] - Analysis of PIK3R4 expression in HeLa (Cat # L013V1).
Western Blot: PIK3R4 Antibody (1G12) [H00030849-M03] - Analysis of PIK3R4 expression in transfected 293T cell line by PIK3R4 monoclonal antibody (M03), clone 1G12. Lane 1: PIK3R4 transfected lysatE (153.103 KDa). Lane ...read more
ELISA: PIK3R4 Antibody (1G12) [H00030849-M03] - Detection limit for recombinant GST tagged PIK3R4 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA
Clone
1G12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

PIK3R4 Antibody (1G12) Summary

Immunogen
PIK3R4 (NP_055417, 1259 a.a. ~ 1358 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AGSDMKIRFWDLAYPERSYVVAGSTSSPSVSYYRKIIEGTEVVQEIQNKQKVGPSDDTPRRGPESLPVGHHDIITDVATFQTTQGFIVTASRDGIVKVWK
Specificity
PIK3R4 - phosphoinositide-3-kinase, regulatory subunit 4, p150
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PIK3R4
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate, transfected lysate and recombinant protein for western blot. It has also been used for ELISA.
Publications
Read Publications using H00030849-M03.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PIK3R4 Antibody (1G12)

  • EC 2.7.11.1
  • MGC102700
  • p150
  • phosphatidylinositol 3-kinase-associated p150
  • Phosphoinositide 3-kinase adaptor protein
  • phosphoinositide 3-kinase regulatory subunit 4
  • phosphoinositide-3-kinase, regulatory subunit 4
  • phosphoinositide-3-kinase, regulatory subunit 4, p150
  • PI3-kinase p150 subunit
  • PI3-kinase regulatory subunit 4
  • PIK3R4
  • VPS 15
  • VPS15

Background

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PIK3R4 Antibody (H00030849-M03)(3)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional PIK3R4 Products

Array H00030849-M03

Research Areas for PIK3R4 Antibody (H00030849-M03)

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Blogs on PIK3R4

There are no specific blogs for PIK3R4, but you can read our latest blog posts.
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Bioinformatics

Gene Symbol PIK3R4
Entrez
OMIM
Uniprot