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PIGP Antibody

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Western Blot: PIGP Antibody [NBP1-84294] - Analysis in control (vector only transfected HEK293T lysate) and PIGP over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Western Blot: PIGP Antibody [NBP1-84294] - Analysis in control (vector only transfected HEK293T lysate) and PIGP over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: PIGP Antibody [NBP1-84294] - Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
Immunohistochemistry-Paraffin: PIGP Antibody [NBP1-84294] - Staining of human stomach shows strong membranous positivity in parietal cells.
Western Blot: PIGP Antibody [NBP1-84294] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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PIGP Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: NMMSTSPLDSIHTITDNYAKNQQQKKYQEEAIPALRDISISEVNQMFFLAAKELYTK
Specificity
Specificity of human PIGP antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PIGP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.04-0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PIGP Protein (NBP1-84294PEP)
Publications
Read Publication using NBP1-84294.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (86%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PIGP Antibody

  • class P
  • DCRCDown syndrome critical region protein C
  • Down syndrome critical region protein 5
  • DSCR5DCRC-S
  • DSCRC
  • DSRC
  • EC 2.4.1.198
  • phosphatidylinositol glycan anchor biosynthesis, class P
  • Phosphatidylinositol-glycan biosynthesis class P protein
  • phosphatidylinositol-n-acetylglucosaminyltranferase subunit
  • PIG-P

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-24653
Western Blot: GNPDA1 Antibody [NBP2-24653] - analysis of GNPDA1 in human spleen lysate in the 1) absence and 2) presence of immunizing peptide 3) mouse spleen and 4) rat spleen using GNPDA1 antibody at 1.0 ug/ml, 0.5 ug/ml and 0.5 ug/ml, respectively.
Immunohistochemistry-Paraffin: GNPDA1 Antibody [NBP2-24653] - Formalin-fixed paraffin-embedded human kidney tissue stained with GNPDA1 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Glucose 6
phosphate isomerase A ...
NBP2-02541
Western Blot: Glucose 6 phosphate isomerase Antibody (2D2) [NBP2-02541] Analysis of extracts (35ug) from 9 different cell lines by using anti-Glucose 6 phosphate isomerasemonoclonal antibody.
Immunocytochemistry/Immunofluorescence: Glucose 6 phosphate isomerase Antibody (2D2) [NBP2-02541] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Glucose 6 phosphate isomerase.
Immunohistochemistry-Paraffin: Glucose 6 phosphate isomerase Antibody (2D2) [NBP2-02541] - Staining of paraffin-embedded Human prostate tissue using anti-Glucose 6 phosphate isomerasemouse monoclonal antibody.
Species: Hu, Mu, Pm
Applications: WB, ICC/IF, IHC, IHC-P
PIGH Antibody
NBP1-83342
Western Blot: PIGH Antibody [NBP1-83342] - Analysis in control (vector only transfected HEK293T lysate) and PIGH over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry: PIGH Antibody [NBP1-83342] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: PIGH Antibody [NBP1-83342] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.
Species: Hu
Applications: WB, IHC, IHC-P
PIGC Antibody
NBP1-86283
Western Blot: PIGC Antibody [NBP1-86283] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: SK-MEL-30
Immunohistochemistry: PIGC Antibody [NBP1-86283] - Staining of human colon shows moderate cytoplasmic and membranous positivity in glandular cells.
Species: Hu
Applications: WB, IHC, IHC-P
Src [p Tyr419]
Antibody [Unconjugate
AF2685
Western blot shows lysates of MDA‑MB‑468 human breast cancer cell line and HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 50 μM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Src (Y419) at approximately 56 - 61 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of MBA‑MB‑468 human breast cancer cell line untreated (-) or treated (+) with 50 µM Pervanadate (PV) for 10 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Src (Y419) at approximately 64 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Src phosphorylated at Y419 was detected in immersion fixed paraffin-embedded sections of human colon using Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(1 Review)
(5 Publications)
Species: Hu
Applications: WB, Simple Western, IHC
PIGA Antibody
NBP2-19827
Western Blot: PIGA Antibody [NBP2-19827] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE gel, diluted at 1:1000.
Species: Mu
Applications: WB
PIGQ Antibody (2B7)
H00009091-M02
Western Blot: PIGQ Antibody (2B7) [H00009091-M02] - Analysis of PIGQ expression in transfected 293T cell line by PIGQ monoclonal antibody (M02), clone 2B7.Lane 1: PIGQ transfected lysate(84.1 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: PIGQ Antibody (2B7) [H00009091-M02] - Analysis of monoclonal antibody to PIGQ on formalin-fixed paraffin-embedded human placenta. Antibody concentration 4 ug/ml.
Sandwich ELISA: PIGQ Antibody (2B7) [H00009091-M02] - Detection limit for recombinant GST tagged PIGQ is approximately 0.1ng/ml as a capture antibody.
Species: Hu
Applications: WB, ELISA, IHC, IHC-P, S-ELISA
Peripherin 2/PRPH2
Antibody
NBP1-86687
Western Blot: Peripherin 2/PRPH2 Antibody [NBP1-86687] - Analysis in control (vector only transfected HEK293T lysate) and PRPH2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: Peripherin 2/PRPH2 Antibody [NBP1-86687] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Peripherin 2/PRPH2 Antibody [NBP1-86687] - Staining of retina shows strong cytoplasmic positivity in photoreceptors.
Species: Hu
Applications: WB, IHC, IHC-P
KMT2B Antibody
NB300-864
Immunohistochemistry-Paraffin: KMT2B Antibody [NB300-864] - (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: KMT2B Antibody [NB300-864] - (5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, PEP-ELISA
Dehydrodolichyl
Diphosphate Synthase ...
NBP1-84975
Immunohistochemistry-Paraffin: Dehydrodolichyl Diphosphate Synthase Antibody [NBP1-84975] - Staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.
(1 Publication)
Species: Hu
Applications: IHC, IHC-P
DHPS Antibody
NBP1-82648
Western Blot: DHPS Antibody [NBP1-82648] - Analysis in control (vector only transfected HEK293T lysate) and DHPS over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: DHPS Antibody [NBP1-82648] - Staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DHPS Antibody [NBP1-82648] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.
(1 Publication)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
DPM2 Antibody
NBP2-84818
Western Blot: DPM2 Antibody [NBP2-84818] - Host: Rabbit. Target Name: DPM2. Sample Tissue: Human HepG2 Whole Cell lysates. Antibody Dilution: 1ug/ml
Species: Hu
Applications: WB
c-Abl Antibody
[Unconjugated]
AF5414
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF‑7 human breast cancer cell line, and MDA‑MB‑453 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human c-Abl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5414) followed by HRP-conjugated Anti‑Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for c-Abl at approximately 135 - 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for c‑Abl at approximately 149 kDa (as indicated) using 10 µg/mL of Goat Anti-Human c‑Abl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5414) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
(2 Reviews)
(1 Publication)
Species: Hu
Applications: WB, Simple Western
Glypican 4 Antibody
NBP1-87697
Immunocytochemistry/Immunofluorescence: Glypican 4 Antibody [NBP1-87697] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Glypican 4 Antibody [NBP1-87697] - Staining of human tonsil shows negative positivity in non-germinal center cells as expected.
Immunohistochemistry-Paraffin: Glypican 4 Antibody [NBP1-87697] - Staining of human kidney shows strong positivity in cytoplasm granular in cells in tubules.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
KMT2D Antibody
NBP1-89123
Immunocytochemistry/Immunofluorescence: KMT2D Antibody [NBP1-89123] - Staining of human cell line A-431 shows localization to nucleus, plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: KMT2D Antibody [NBP1-89123] - Staining of human endometrium shows moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: KMT2D Antibody [NBP1-89123] - Staining of human cervix, uterine shows weak nuclear positivity in squamous epithelial cells.
(2 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
KCNE1 Antibody
(5B12)
H00003753-M01
Western Blot: KCNE1 Antibody (5B12) [H00003753-M01] - Analysis of KCNE1 over-expressed 293 cell line, cotransfected with KCNE1 Validated Chimera RNAi ( Cat # H00003753-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with KCNE1 monoclonal antibody (M01), clone 5B12 (Cat # H00003753-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: KCNE1 Antibody (5B12) [H00003753-M01] - analysis of KCNE1 expression in transfected 293T cell line by KCNE1 monoclonal antibody (M01), clone 5B12. Lane 1: KCNE1 transfected lysate (14.7 KDa). Lane 2: Non-transfected lysate.
ELISA: KCNE1 Antibody (5B12) [H00003753-M01] - Detection limit for recombinant GST tagged KCNE1 is approximately 3ng/ml as a capture antibody.
(2 Publications)
Species: Hu, Rt, Ca, Gp, Pm
Applications: WB, ELISA, ICC/IF, RNAi
IRS1 Antibody
NB100-82001
Western Blot: IRS1 Antibody [NB100-82001] - Analysis of extracts from 293 cells using IRS-1 (Ab-636) antibody
Immunocytochemistry/Immunofluorescence: IRS1 Antibody [NB100-82001] - Staining of methanol-fixed Hela cells using IRS-1 (Ab-636) antibody
Immunohistochemistry-Paraffin: IRS1 Antibody [NB100-82001] - Analysis of breast carcinoma tissue, using IRS-1 (Ab-636) antibody.
(3 Publications)
Species: Hu, Mu, Rt, Po
Applications: WB, ICC/IF, IHC, IHC-P
Recombinant Human
DSCR6 GST (N-Term) Pr ...
H00053820-P01
12.5% SDS-PAGE Stained with Coomassie Blue.
Species: Hu
Frizzled-7
Antibody [Unconjugate
AF198
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Frizzled‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF198) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Frizzled‑7 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Frizzled‑7 was detected in immersion fixed frozen sections of embryonic mouse gastrointestinal tract (11 d.p.c.) using 15 µg/mL Goat Anti-Mouse Frizzled‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF198) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
(5 Publications)
Species: Mu
Applications: WB, IHC
PIGP Antibody
NBP1-84294
PIGP Antibody
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for PIGP Antibody (NBP1-84294)(1)


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Bioinformatics

Gene Symbol PIGP