Frizzled-7 Antibody [Unconjugated]

Images

 
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Frizzled‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF198) ...read more
Frizzled‑7 was detected in immersion fixed frozen sections of embryonic mouse gastrointestinal tract (11 d.p.c.) using 15 µg/mL Goat Anti-Mouse Frizzled‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

Frizzled-7 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-7
Gln33-Leu185
Accession # Q61090
Specificity
Detects mouse Frizzled-7 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse (rm) Frizzled-2 and less than 5% cross-reactivity with rmFrizzled-3, rmFrizzled-4, rhFrizzled-5, rmFrizzled-6, rmFrizzled-8, and rmFrizzled-9 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FZD7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF198 in the following applications:

  • ICC
    2 publications
  • IHC
    2 publications
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Frizzled-7 Antibody [Unconjugated]

  • frizzled (Drosophila) homolog 7
  • frizzled homolog 7 (Drosophila)
  • Frizzled, drosophila, homolog of, 7
  • Frizzled7
  • Frizzled-7
  • fz-7
  • FZD7
  • FzE3frizzled-7
  • hFz7

Background

The Wnt genes encode a large family of glycoproteins that are essential in development and tissue maintenance (1). Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway (2). The predicted structure of Frizzled proteins is similar among all family members, containing a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain (CRD), a variable-length linker region, a seven-pass transmembrane region, and a variable-length C-terminal cytoplasmic domain. The CRD, which comprises the Wnt binding site, spans about 130 amino acid residues and contains ten invariant cysteine residues. Mouse Frizzled-7 contains 572 amino acid residues and shares 99% identity with the human orthologue in the CRD (3, 4). Frizzled-7 mRNA has been detected in relatively large amounts in adult skeletal muscle, plancenta and heart, and fetal kidney and lung (3). Several Frizzled-dependent signaling pathways exist (2). Their activation depends on the Wnt ligand and the cell context. Frizzled-7 can activate canonical Wnt/ beta -Catenin signaling, as well as a non-canonical pathway involved in tissue morphogenesis (4, 5).

  1. Wodarz, A. and R. Nusse (1998) Annu. Rev. Cell Dev. Biol. 14:59.
  2. Hsieh, J-C. (2004) Front. Biosci. 9:1333.
  3. Sagara, N. et al. (1998) Biochem. Biophys. Res. Commun. 252:117.
  4. Sheldahl, L.C. et al. (1999) Curr. Biol. 9:695.
  5. Medina, A. et al. (2000) Mech. Dev. 92:227.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF1120
Species: Mu
Applications: WB, IHC
AF1617
Species: Hu
Applications: WB
NBP1-54467
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC, IF
NLS5103
Species: Hu, Bv, Ca
Applications: IHC-P, ICC
NBP1-20920
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA
MAB194
Species: Hu, Mu
Applications: WB, Flow, CyTOF-ready, ICC
NB100-40793
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA
AF1526
Species: Mu
Applications: WB, Flow, IHC, CyTOF-ready
NB100-2439
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA
645-WN/CF
Species: Hu, Mu
AF1001
Species: Mu
Applications: IHC
NBP2-23659
Species: Hu, Mu, Pm
Applications: WB
NBP2-24519
Species: Hu, Mu, Bv, Ca, Ch, Eq, Pm
Applications: WB
AF1505
Species: Hu
Applications: WB, Flow, CyTOF-ready
NBP1-54467
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC, IF
NBP1-89679
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF198
Species: Mu
Applications: WB, IHC

Publications for Frizzled-7 Antibody (AF198)(5)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC, IHC, WB.


Filter By Application
ICC
(2)
IHC
(2)
WB
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(2)
Rat
(1)
All Species

Reviews for Frizzled-7 Antibody (AF198) (0)

There are no reviews for Frizzled-7 Antibody (AF198). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Frizzled-7 Antibody (AF198) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for Frizzled-7 Antibody (AF198)

Discover related pathways, diseases and genes to Frizzled-7 Antibody (AF198). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Frizzled-7 Antibody (AF198)

Discover more about diseases related to Frizzled-7 Antibody (AF198).
 

Pathways for Frizzled-7 Antibody (AF198)

View related products by pathway.

PTMs for Frizzled-7 Antibody (AF198)

Learn more about PTMs related to Frizzled-7 Antibody (AF198).

Blogs on Frizzled-7

There are no specific blogs for Frizzled-7, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Frizzled-7
6178-FZ-050

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Frizzled-7 Antibody [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol FZD7
Uniprot