PIGH Antibody - BSA Free

Western Blot: PIGH Antibody [NBP1-83342] - Analysis in control (vector only transfected HEK293T lysate) and PIGH over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry: PIGH Antibody [NBP1-83342] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: PIGH Antibody [NBP1-83342] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.

Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.

Staining of human skeletal muscle shows very weak positivity in myocytes as expected.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PIGH Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PIGH Antibody - BSA Free (NBP1-83342) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: KIDQETLLIIDSLGIQMTSSYASGKESTTFIEMGKVKDIVINEAIYMQKVIYYLCILLKDPVEPHGISQVVPVFQSAKPRLDCLIEVYRSCQEILAHQKATSTS
• Predicted Species: Mouse (91%), Rat (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PIGH
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:10-1:20
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended..
• Control Peptide:

  PIGH Protein (NBP1-83342PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PIGH Antibody - BSA Free

• EC 2.4.1.198
• GPI-H
• phosphatidylinositol glycan anchor biosynthesis, class H
• phosphatidylinositol glycan, class H
• phosphatidylinositol N-acetylglucosaminyltransferase subunit H
• Phosphatidylinositol-glycan biosynthesis class H protein
• phosphatidylinositol-glycan biosynthesis, class H protein
• PIG-H

Background

PIGH encodes an endoplasmic reticulum associated protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI anchor is a glycolipid found on many blood cells and which serves to anchor proteins to the cell surface. The protein encoded by this gene is a subunit of the GPI N-acetylglucosaminyl (GlcNAc) transferase that transfers GlcNAc to phosphatidylinositol (PI) on the cytoplasmic side of the endoplasmic reticulum. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PIGH