PIEZO1 Antibody

Images

 
Western Blot: PIEZO1 Antibody [NBP1-78537] - Analysis of MCF7 cell lysate using PIEZ01 antibody (NBP1-78537) at 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: PIEZO1 Antibody [NBP1-78537] - PIEZO1 antibody was tested in A431 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Immunohistochemistry: PIEZO1 Antibody [NBP1-78537] - Analysis of PIEZ01 in mouse epidermis using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.11 mg/ml

PIEZO1 Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human PIEZO1 protein (between residues 1300-1350) [UniProt Q92508]
Localization
Multi-pass membrane protein: Endoplasmic reticulum, Golgi intermediate compartment, Cell membrane.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PIEZO1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • Immunocytochemistry/Immunofluorescence 1:25
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
Application Notes
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Control Peptide
PIEZO1 Peptide (NBP1-78537PEP)
Publications
Read Publications using
NBP1-78537 in the following applications:

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.11 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PIEZO1 Antibody

  • FAM38A
  • member A
  • membrane protein induced by beta-amyloid treatment
  • Mib
  • piezo-type mechanosensitive ion channel component 1

Background

Piezo1 (Mib, FAM38A) is a mechanosensitive ion channel which responds to mechanically activated currents in numerous cells, including the brain, bladder, colon, kidney, lung, and skin. However, mRNA expression of Piezo1 is weak in the DRG, unlike Piezo2. The expression of Piezo1 is induced in Alzheimer's associated senile plaques (PMID 16854388), as well as integrin activation through R-Ras signaling (PMID 20016066). R-Ras is recruited to the endoplasmic reticulum which increases calcium release, leading to the activation of the protease calpain. Piezo proteins are fairly novel proteins, and whose sequences are not akin to other ion channels or proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PIEZO1 Antibody (NBP1-78537)(3)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PIEZO1 Antibody (NBP1-78537) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-78537AF405
Alexa Fluor 488 Labeled NBP1-78537AF488
Alexa Fluor 647 Labeled NBP1-78537AF647
Alexa Fluor 700 Labeled NBP1-78537AF700
Biotin Labeled NBP1-78537B
DyLight 405 Labeled NBP1-78537V
DyLight 488 Labeled NBP1-78537G
DyLight 550 Labeled NBP1-78537R
DyLight 650 Labeled NBP1-78537C
FITC Labeled NBP1-78537F

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Bioinformatics Tool for PIEZO1 Antibody (NBP1-78537)

Discover related pathways, diseases and genes to PIEZO1 Antibody (NBP1-78537). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for PIEZO1 Antibody (NBP1-78537)

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Blogs on PIEZO1.

Touch Infographic: From Touch Receptors to the Brain
The body contains thousands of receptors and nerves which allow us to experience the sense of touch, also referred to as tactile perception. The somatosensory system allows organisms to perceive and decode a wide range of tactile stimuli to allow for...  Read full blog post.

PIEZO1: A Mechanosensitive Ion Channel Protein
PIEZO1, and its close homologue PIEZO2, are mechanosensitive ion channel proteins. Mechanosensitive ion channels couple protein conformation to the mechanics of the surrounding membrane, and switch between a closed state and an open state in response...  Read full blog post.

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Bioinformatics

Gene Symbol PIEZO1
Entrez
Uniprot