PIEZO1 Antibody

Western Blot: PIEZO1 Antibody [NBP1-78537] - Analysis of MCF7 cell lysate using PIEZ01 antibody (NBP1-78537) at 2 ug/mL.

Immunocytochemistry/Immunofluorescence: PIEZO1 Antibody [NBP1-78537] - PIEZO1 antibody was tested in A431 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry: PIEZO1 Antibody [NBP1-78537] - Analysis of PIEZ01 in mouse epidermis using DAB with hematoxylin counterstain.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.11 mg/ml

PIEZO1 Antibody Summary

• Immunogen: A synthetic peptide made to an internal portion of the human PIEZO1 protein (between residues 1300-1350) [UniProt Q92508]
• Localization: Multi-pass membrane protein: Endoplasmic reticulum, Golgi intermediate compartment, Cell membrane.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PIEZO1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL
  • Immunocytochemistry/Immunofluorescence 1:25
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
• Application Notes: Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1.11 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PIEZO1 Antibody

• FAM38A
• member A
• membrane protein induced by beta-amyloid treatment
• Mib
• piezo-type mechanosensitive ion channel component 1

Background

Piezo1 (Mib, FAM38A) is a mechanosensitive ion channel which responds to mechanically activated currents in numerous cells, including the brain, bladder, colon, kidney, lung, and skin. However, mRNA expression of Piezo1 is weak in the DRG, unlike Piezo2. The expression of Piezo1 is induced in Alzheimer's associated senile plaques (PMID 16854388), as well as integrin activation through R-Ras signaling (PMID 20016066). R-Ras is recruited to the endoplasmic reticulum which increases calcium release, leading to the activation of the protease calpain. Piezo proteins are fairly novel proteins, and whose sequences are not akin to other ion channels or proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PIEZO1 Antibody (NBP1-78537)(8)

Publications using NBP1-78537

• Morozumi W, Inagaki S, Iwata Y, et al. Piezo channel plays a part in retinal ganglion cell damage Exp. Eye Res. Dec 23 2019 [PMID: 31874142] (IHC, Mouse)
• Du G, Li L, Zhang X, et al. Roles of TRPV4 and piezo channels in stretch-evoked Ca2+ response in chondrocytes Exp. Biol. Med. (Maywood) Dec 2 2019 [PMID: 31791130] (WB)
• Etem EO, Ceylan GG, Ozaydın S The increased expression of Piezo1 and Piezo2 ion channels in human and mouse bladder carcinoma. Adv Clin Exp Med. [PMID: 30010255] (IHC-P, Human)
• Jiang Long, Zhao Yi-Ding, Chen Wei-Xiang. The Function of the Novel Mechanical Activated Ion Channel Piezo1 in the Human Osteosarcoma Cells. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research 2017 [PMID: 29065102] (ICC/IF, WB, Human)
• Zhang HN. Piezo1 protein induces the apoptosis of human osteoarthritis-derived chondrocytes by activating caspase-12, the signaling marker of ER stress. Int J Mol Med. 2018 Feb 22 [PMID: 28731145] (ICC/IF, Human)
  
  Details:
  PIEZO1 antibody from Novus was used to determine the expression and location of the PIEZO1 protein in OA-derived chondrocytes and showed PIEZO1 is largely confined to the cell membrane and nucleus of the chondrocytes
• Lee K, Guevarra M, Nguyen A et al. The primary cilium functions as a mechanical and calcium signaling nexus. Ciliajournal 2015 May 29 [PMID: 26029358] (Mouse)
  
  Details:
  The PIEZO 1 antibody was used to detect PIEZO1 in the primary cilia and the membrane of MLO-YR mouse cells
• Li X, Liu Q, Zhang H Effects of mechanosensitive ion channel Piezo1 on Osteoarthritic articular chondrocytes Chronic Diseases Prevention Review (ICC/IF, Human)
• Miyazaki A, Sugimoto A, Yoshizaki K et al. Coordination of WNT signaling and ciliogenesis during odontogenesis by piezo type mechanosensitive ion channel component 1. Sci Rep. 2019 Oct 14 [PMID: 31611621] (IHC-P, Human)

FAQs for PIEZO1 Antibody (NBP1-78537). (Showing 1 - 1 of 1 FAQs).

1. What is the expected molecular weight of the detected band in WB (mouse protein) in NBP1-78537? Has there been detection at lower molecular weight?
   • This human protein has at least one isoform with the major one has 2,521 a.a. with the calculated size of 286,790 Da.  If considering the post-translational modifications (phosphorylated a.a. and glycosylations), the apparent size could be considerable larger (https://www.uniprot.org/uniprot/Q92508#sequences).  Above website also shows some potential isoforms with the much smaller sizes (in 300 a.a or less).  Whereas the counterpart in mouse has the major isoform of 2,547 a.a with 292,002 Da, and 4 potential isoforms (ranging from 1000 a.a. - 2500+ a.a.; https://www.uniprot.org/uniprot/E2JF22#sequences) [however it doesn't mean that there is no other smaller ones like human; just that probably no scientist has looked for them, because the proteins in the high mammals are very conserved]. 1) In terms of detecting the smaller PIEZO1 species, the WB image (fig# 1) done by our manufacturing lab on the MCF7 cell lysate shows that many smaller bands below the expected PIEZO1 band (above 251 kDa).   2) I think they could not assign any of the smaller bands to the protein target was because a, for expressing the large proteins in vivo and in vitro, they intend to be pre-mature terminated of the chain synthesis.  Therefore the shorter chain(s) can vary in size(s) in the different cases.   3) I checked online and found that https://www.frontiersin.org/articles/10.3389/fnagi.2018.00332/full shows rat PIEZO1 has many smaller bands (Fig 1); seems that it was a wide-spread observation.4) All rabbit polyclonal antibodies, more or less, have some backgrounds/non-specific bands, because of the nature of the host.  Only performing the steps to block the membranes well and incubate the Abs at room temp for 1 hr (or less) [and use less Ab as possible] to reduce the backgrounds/non-specific bands.

Bioinformatics

• Gene Symbol: PIEZO1
• Entrez:
  • 9780(Human)
• Uniprot:
  • Q92508(Human)