Western Blot: PIEZO1 Antibody [NBP1-78537] - Western blot analysis of MCF7 cell lysate using PIEZ01 antibody (NBP1-78537) at 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: PIEZO1 Antibody [NBP1-78537] - PIEZO1 antibody was tested in A431 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Immunohistochemistry: PIEZO1 Antibody [NBP1-78537] - Analysis of PIEZ01 in mouse epidermis using DAB with hematoxylin counterstain.
This PIEZO1 antibody is useful for Western blot, ICC and IHC-paraffin embedded sections. In ICC/IF, membrane staining is observed in A431 cells. In IHC-P, membrane and cytoplasmic staining staining is seen in mouse epidermal tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.05% Sodium Azide
Immunogen affinity purified
Alternate Names for PIEZO1 Antibody
membrane protein induced by beta-amyloid treatment
piezo-type mechanosensitive ion channel component 1
Piezo1 (Mib, FAM38A) is a mechanosensitive ion channel which responds to mechanically activated currents in numerous cells, including the brain, bladder, colon, kidney, lung, and skin. However, mRNA expression of Piezo1 is weak in the DRG, unlike Piezo2. The expression of Piezo1 is induced in Alzheimer's associated senile plaques (PMID 16854388), as well as integrin activation through R-Ras signaling (PMID 20016066). R-Ras is recruited to the endoplasmic reticulum which increases calcium release, leading to the activation of the protease calpain. Piezo proteins are fairly novel proteins, and whose sequences are not akin to other ion channels or proteins.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our PIEZO1 Antibody and receive a gift card or discount.