PIBF1 Antibody

Western Blot: PIBF1 Antibody [NBP2-19823] - Sample (30 ug of whole cell lysate) A: HCT116 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: PIBF1 Antibody [NBP2-19823] - Analysis of methanol-fixed HCT116, using antibody at 1:500 dilution.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PIBF1 Antibody Summary

• Description: Novus Biologicals Rabbit PIBF1 Antibody (NBP2-19823) is a polyclonal antibody validated for use in WB and ICC/IF. Anti-PIBF1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human PIBF1. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PIBF1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 90 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for PIBF1 Antibody

• C13orf24
• chromosome 13 open reading frame 24
• KIAA1008
• PIBF
• PIBF1
• progesterone immunomodulatory binding factor 1
• progesterone-induced blocking factor 1
• progesterone-induced-blocking factor 1
• RP11-505F3.1

Background

Mediator of progesterone that by acting on the phospholipase A2 enzyme interferes with arachidonic acid metabolism, induces a Th2 biased immune response, and by controlling NK activity exerts an anti-abortive effect.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for PIBF1 Antibody (NBP2-19823)(1)

Publication using NBP2-19823

• Wheway G, Schmidts M, Mans DA et al. An siRNA-based functional genomics screen for the identification of regulators of ciliogenesis and ciliopathy genes. Nat Cell Biol 2015-08-01 [PMID: 26167768] (ICC/IF, Human)

Reviews for PIBF1 Antibody (NBP2-19823) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Mouse.

reviewed by: Kasia Szymanska WB Human 10/30/2013

Summary

• Application: Western Blot
• Sample Tested: IMCD3, hTertRPE1, HDF, SHSY5Y whole cell lysates
• Species: Human
• Lot: 40442

reviewed by: Gabrielle Wheway IF Mouse 10/14/2013

Summary

• Application: Immunofluorescence
• Sample Tested: Mouse inner medullary collecting duct (IMCD3) cell line
• Species: Mouse
• Lot: 40442

Bioinformatics

• Gene Symbol: PIBF1
• Uniprot:
  • Q8WXW3()