PI 3-Kinase p85 alpha Antibody (A3-D0)

Images

 
Western Blot: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Analysis on different cell lysates using anti-PI3-kinase p85 subunit alpha Mouse mAb. Positive control: Lane 1: U-937 Lane 2: Raji
Immunocytochemistry/ Immunofluorescence: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Staining of MCF-7 cells using anti-PI3-kinase p85 subunit alpha Mouse mAb.
Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Analysis of paraffin- embedded mouse skeletal muscle tissue using anti-PI3-kinase p85 subunit alpha Mouse mAb.
Flow Cytometry: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Analysis of MCF-7 cells with PI3-kinase p85 subunit alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without ...read more
Immunocytochemistry/ Immunofluorescence: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Staining of Hela cells using anti-PI3-kinase p85 subunit alpha Mouse mAb.
Immunocytochemistry/ Immunofluorescence: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Staining of SH-SY-5Y cells using anti-PI3-kinase p85 subunit alpha Mouse mAb.
Immunocytochemistry/ Immunofluorescence: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Staining of PANC-1 cells using anti-PI3-kinase p85 subunit alpha Mouse mAb.
Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Analysis of paraffin- embedded human breast carcinoma tissue using anti-PI3-kinase p85 subunit alpha Mouse mAb.
Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Analysis of paraffin- embedded human colon cancer tissue using anti-PI3-kinase p85 subunit alpha Mouse mAb.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
A3-D0
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
2 mg/ml

Order Details

PI 3-Kinase p85 alpha Antibody (A3-D0) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
This recombinant PI 3-Kinase p85 alpha Antibody (A3-D0) was developed against recombinant protein (SwissProt: P27986 Human)
Localization
Cytosol, Membrane, Nucleus
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PIK3R1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:2000
Theoretical MW
84 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
2 mg/ml
Purity
Protein A purified

Alternate Names for PI 3-Kinase p85 alpha Antibody (A3-D0)

  • AGM7
  • GRB1
  • IMD36
  • p85
  • p85-ALPHA
  • Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha
  • phosphatidylinositol 3-kinase regulatory subunit alpha
  • phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)
  • phosphatidylinositol 3-kinase-associated p-85 alpha
  • phosphoinositide-3-kinase regulatory subunit alpha
  • phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
  • PI 3Kinase p85 alpha
  • PI 3-Kinase p85 alpha
  • PI3K regulatory subunit alpha
  • PI3-kinase regulatory subunit alpha
  • PI3-kinase subunit p85-alpha
  • PIK3R1
  • PtdIns-3-kinase regulatory subunit alpha
  • PtdIns-3-kinase regulatory subunit p85-alpha

Background

Phosphatidylinositol 3-kinase (PI3K) p85 alpha is a regulatory subunit that heterodimerizes with a catalytic subunit to form a Class IA PI3K enzyme complex, which plays an important role in the immune system (1,2). The P13K pathway is involved in many diverse processes including growth, metabolism, proliferation, and survival (2). PI3Ks are typically activated by cytokine receptors and are responsible for phosphorylation of the 3'-hydroxyl group of PI and its derivatives (3). p85 alpha is one of five regulatory subunit proteins which also includes p55 alpha, p50 alpha, p85 beta, and p55 gamma, and can bind to two of the three catalytic subunits (p110 alpha or p110 delta) (1,2). p85 alpha, p55 alpha, and p50 alpha proteins are all synthesized by the same PIK3R1 gene by alternative splicing (1). Structurally, PI 3-Kinase p85 alpha contains SRC homology 3 (SH3 domain), followed by a Bcr homology (BH) domain flanked by two proline-rich regions, then a N-terminal SH2 domain, an inter-SH2 domain, and C-terminal SH2 domain (1,2). PI 3-Kinase p85 alpha protein consists of 724 amino acids (aa) in length with a theoretical molecular weight of 83.5 kDa (2,4). The primary role for the p85 subunit is interaction with cell surface receptors and acts as an adapter for the stabilization and recruitment of the p110 catalytic subunit to the plasma membrane (1,3). Additionally, p85 has been shown to function in both interleukin-2 receptor (IL2R) and erythropoietin receptor (EpoR) endocytosis (3).

The PI3K pathway functions in a broad range of cellular processes, so it is understandable that pathway dysfunction can lead to an array of diseases and disorders (2,5). Elevated PI3K signaling is a key feature of many cancers (5). PI3K pathway dysregulation has also been implicated in neurological, metabolic, and cardiovascular disorders (5). Furthermore, both overactivation or under-activation of the PI3K delta (p85 alpha subunit + p110 delta subunit) pathway has been shown to cause immunodeficiency and pathologies related to immune system dysfunction (2). Therapeutics to target the PI3K pathway and treat related cancers include PI3K inhibitors and, specifically, isoform-selective inhibitors which have a lot of promise when used as part of a combination therapy (5).

References

1. Okkenhaug, K., & Vanhaesebroeck, B. (2001). New responsibilities for the PI3K regulatory subunit p85 alpha. Science's STKE : signal transduction knowledge environment. https://doi.org/10.1126/stke.2001.65.pe1

2. Nunes-Santos, C. J., Uzel, G., & Rosenzweig, S. D. (2019). PI3K pathway defects leading to immunodeficiency and immune dysregulation. The Journal of allergy and clinical immunology. https://doi.org/10.1016/j.jaci.2019.03.017

3. Chen, P. H., Yao, H., & Huang, L. J. (2017). Cytokine Receptor Endocytosis: New Kinase Activity-Dependent and -Independent Roles of PI3K. Frontiers in endocrinology. https://doi.org/10.3389/fendo.2017.00078

4. Uniprot (P27986)

5. Fruman, D. A., Chiu, H., Hopkins, B. D., Bagrodia, S., Cantley, L. C., & Abraham, R. T. (2017). The PI3K Pathway in Human Disease. Cell. https://doi.org/10.1016/j.cell.2017.07.029

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PIK3R1