| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2998-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22212-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2998-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89731-25ul | |||
![Western Blot: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Analysis on different cell lysates using anti-PI3-kinase p85 subunit alpha Mouse mAb. Positive control: Lane 1: U-937 Lane 2: Raji](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-A3-D0-Western-Blot-NBP2-67058-img0006.jpg "Western Blot: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Analysis on different cell lysates using anti-PI3-kinase p85 subunit alpha Mouse mAb. Positive control: Lane 1: U-937 Lane 2: Raji")
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Staining of MCF-7 cells using anti-PI3-kinase p85 subunit alpha Mouse mAb.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-A3-D0-Immunocytochemistry-Immunofluorescence-NBP2-67058-img0008.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Analysis of paraffin- embedded mouse skeletal muscle tissue using anti-PI3-kinase p85 subunit alpha Mouse mAb.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-A3-D0-Immunohistochemistry-Paraffin-NBP2-67058-img0009.jpg)
![Flow Cytometry: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Analysis of MCF-7 cells with PI3-kinase p85 subunit alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondar](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-A3-D0-Flow-Cytometry-NBP2-67058-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Staining of Hela cells using anti-PI3-kinase p85 subunit alpha Mouse mAb.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-A3-D0-Immunocytochemistry-Immunofluorescence-NBP2-67058-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Staining of SH-SY-5Y cells using anti-PI3-kinase p85 subunit alpha Mouse mAb.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-A3-D0-Immunocytochemistry-Immunofluorescence-NBP2-67058-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Staining of PANC-1 cells using anti-PI3-kinase p85 subunit alpha Mouse mAb.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-A3-D0-Immunocytochemistry-Immunofluorescence-NBP2-67058-img0007.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Analysis of paraffin- embedded human breast carcinoma tissue using anti-PI3-kinase p85 subunit alpha Mouse mAb.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-A3-D0-Immunohistochemistry-Paraffin-NBP2-67058-img0004.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Analysis of paraffin- embedded human colon cancer tissue using anti-PI3-kinase p85 subunit alpha Mouse mAb.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-A3-D0-Immunohistochemistry-Paraffin-NBP2-67058-img0005.jpg)
![Western Blot: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Analysis of PI 3 Kinase p85 alpha on different lysates using anti-PI 3 Kinase p85 alpha antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: Raji](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Western-Blot-NBP2-67488-img0010.jpg "Western Blot: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Analysis of PI 3 Kinase p85 alpha on different lysates using anti-PI 3 Kinase p85 alpha antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: Raji")
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Staining PI 3 Kinase p85 alpha in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Immunocytochemistry-Immunofluorescence-NBP2-67488-img0005.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Analysis of paraffin-embedded mouse kidney tissue using anti-PI 3 Kinase p85 alpha antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Immunohistochemistry-Paraffin-NBP2-67488-img0009.jpg)
![Flow Cytometry: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Analysis of HepG2 cells with PI 3 Kinase p85 alpha antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antib](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Flow-Cytometry-NBP2-67488-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Staining PI 3 Kinase p85 alpha in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Immunocytochemistry-Immunofluorescence-NBP2-67488-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Staining PI 3 Kinase p85 alpha in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Immunocytochemistry-Immunofluorescence-NBP2-67488-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Staining PI 3 Kinase p85 alpha in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Immunocytochemistry-Immunofluorescence-NBP2-67488-img0004.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Analysis of paraffin-embedded human kidney tissue using anti-PI 3 Kinase p85 alpha antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Immunohistochemistry-Paraffin-NBP2-67488-img0006.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Analysis of paraffin-embedded human liver cancer tissue using anti-PI 3 Kinase p85 alpha antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Immunohistochemistry-Paraffin-NBP2-67488-img0007.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Analysis of paraffin-embedded mouse heart tissue using anti-PI 3 Kinase p85 alpha antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Immunohistochemistry-Paraffin-NBP2-67488-img0008.jpg)
![Western Blot: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Analysis using PI 3 Kinase p85 alpha mAb against human PI 3 Kinase p85 alpha recombinant protein. (Expected MW is 53.4 kDa)](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-6G10-Western-Blot-NBP2-22212-img0007.jpg "Western Blot: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Analysis using PI 3 Kinase p85 alpha mAb against human PI 3 Kinase p85 alpha recombinant protein. (Expected MW is 53.4 kDa)")
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Analysis of HepG2 cells using PI 3 Kinase p85 alpha mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-6G10-Immunocytochemistry-NBP2-22212-img0009.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Analysis of rectum cancer tissues using PI 3 Kinase p85 alpha mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-6G10-Immunohistochemistry-Paraffin-NBP2-22212-img0005.jpg)
![Flow Cytometry: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Analysis of NIH3T3 cells using PI 3 Kinase p85 alpha mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-6G10-Flow-Cytometry-NBP2-22212-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Analysis of HeLa cells using PI 3 Kinase p85 alpha mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-6G10-Immunocytochemistry-NBP2-22212-img0008.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Analysis of cervical cancer tissues using PI 3 Kinase p85 alpha mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-6G10-Immunohistochemistry-Paraffin-NBP2-22212-img0004.jpg)
![ELISA: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-6G10-ELISA-NBP2-22212-img0006.jpg)
![Western Blot: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - Analysis of PIK3R1 expression in transfected 293T cell line by PIK3R1 monoclonal antibody (M01), clone 3A10.Lane 1: PIK3R1 transfected lysate (Predicted MW: 50 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-3A10-Western-Blot-H00005295-M01-img0016.jpg "Western Blot: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - Analysis of PIK3R1 expression in transfected 293T cell line by PIK3R1 monoclonal antibody (M01), clone 3A10.Lane 1: PIK3R1 transfected lysate (Predicted MW: 50 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - PIK3R1 monoclonal antibody (M01), clone 3A10 Analysis of PIK3R1 expression in HeLa.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-3A10-Western-Blot-H00005295-M01-img0012.jpg)
![Western Blot: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - PIK3R1 monoclonal antibody (M01), clone 3A10. Analysis of PIK3R1 expression in PC-12.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-3A10-Western-Blot-H00005295-M01-img0013.jpg)
![Western Blot: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - PIK3R1 monoclonal antibody (M01), clone 3A10. Analysis of PIK3R1 expression in Raw 264.7.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-3A10-Western-Blot-H00005295-M01-img0014.jpg)
![Western Blot: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - PIK3R1 monoclonal antibody (M01), clone 3A10. Analysis of PIK3R1 expression in NIH/3T3.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-3A10-Western-Blot-H00005295-M01-img0015.jpg)
![Proximity Ligation Assay: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - Analysis of protein-protein interactions between EGFR and PIK3R1. HeLa cells were stained with anti-EGFR rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-3A10-Proximity-Ligation-Assay-H00005295-M01-img0007.jpg)
![Proximity Ligation Assay: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - Analysis of protein-protein interactions between FLT1 and PIK3R1. Huh7 cells were stained with anti-FLT1 rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-3A10-Proximity-Ligation-Assay-H00005295-M01-img0008.jpg)
![Proximity Ligation Assay: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - Analysis of protein-protein interactions between CRKL and PIK3R1. Mahlavu cells were stained with anti-CRKL rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-3A10-Proximity-Ligation-Assay-H00005295-M01-img0009.jpg)
![Immunoprecipitation: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - Analysis of PIK3R1 transfected lysate using anti-PIK3R1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PIK3R1 monoclonal antibody.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-3A10-Immunoprecipitation-H00005295-M01-img0010.jpg)
![Sandwich ELISA: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - Detection limit for recombinant GST tagged PIK3R1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-3A10-Sandwich-ELISA-H00005295-M01-img0011.jpg)
![Western Blot: PI 3-Kinase p85 alpha Antibody [NBP2-19815] - Non-transfected (-) and transfected (+) 293T whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3-Kinase p85 alpha antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-Western-Blot-NBP2-19815-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: PI 3-Kinase p85 alpha Antibody [NBP2-19815] - Non-transfected (-) and transfected (+) 293T whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3-Kinase p85 alpha antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody [NBP2-19815] - Paraffin-embedded mouse duodenum. PI3 kinase p85 alpha antibody [N2C1], Internal diluted at 1:500.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-19815-img0008.jpg)
![Western Blot: PI 3-Kinase p85 alpha Antibody [NBP2-19815] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal diluted at 1:500.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-Western-Blot-NBP2-19815-img0006.jpg)
![Immunoprecipitation: PI 3-Kinase p85 alpha Antibody [NBP2-19815] - Jurkat whole cell extract A. Control with 5 ug of preimmune Rabbit IgG B. Immunoprecipitation of PI3 kinase p85 alpha protein by 5 ug PI3 kinase p85 alpha antibody [N2C1], Internal 10 % SDS-PAGE The immunoprecipitated PI3 kinase p85 alpha protein was detected by PI3 kinase p85 alpha antibody [N2C1], Internal diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-Immunoprecipitation-NBP2-19815-img0004.jpg)
![Western Blot: PI 3-Kinase p85 alpha Antibody [NBP2-19815] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI 3-Kinase p85 alpha antibody [N2C1], Internal (NBP2-19815) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19815_rabbit-polyclonal-pi-3-kinase-p85-alpha-antibody-91020242014678.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal (NBP2-19815) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19815_rabbit-polyclonal-pi-3-kinase-p85-alpha-antibody-western-blot-261120251992712.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal (NBP2-19815) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19815_rabbit-polyclonal-pi-3-kinase-p85-alpha-antibody-western-blot-2611202518294918.jpg)
![Western Blot: PI 3-Kinase p85 alpha [p Tyr199, p Tyr467] Antibody [NBP3-13116] -Untreated (-) and treated (+) Raw264.7 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199) antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/antibody/nbp3-13116_rabbit-pi-3-kinase-p85-alpha-p-tyr-199-p-tyr-467-pab-western-blot-4320247318..jpg "Western Blot: PI 3-Kinase p85 alpha [p Tyr199, p Tyr467] Antibody [NBP3-13116] -Untreated (-) and treated (+) Raw264.7 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199) antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Western Blot: PI 3-Kinase p85 alpha [p Tyr199, p Tyr467] Antibody [NBP3-13116] -Untreated (-) and treated (+) C2C12 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199) antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/antibody/nbp3-13116_rabbit-pi-3-kinase-p85-alpha-p-tyr-199-p-tyr-467-pab-western-blot-4320247657..jpg)
![Western Blot: PI 3-Kinase p85 alpha Antibody (7Z9H0) [NBP3-16525] - Western blot analysis of extracts of various cell lines, using PI 3-Kinase p85 alpha Rabbit mAb (NBP3-16525) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-7Z9H0-Western-Blot-NBP3-16525-img0002.jpg "Western Blot: PI 3-Kinase p85 alpha Antibody (7Z9H0) [NBP3-16525] - Western blot analysis of extracts of various cell lines, using PI 3-Kinase p85 alpha Rabbit mAb (NBP3-16525) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: PI 3-Kinase p85 alpha Antibody (3X3T8) [NBP3-16525] - Western blot analysis of various lysates, using PI 3-Kinase p85 alpha Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-16525_rabbit-pi-3-kinase-p85-alpha-mab-3x3t8-52202511365130.jpg)
![Western Blot: PI 3-Kinase p85 alpha Antibody (3C11) [H00005295-M03] - Analysis of PIK3R1 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-3C11-Western-Blot-H00005295-M03-img0010.jpg "Western Blot: PI 3-Kinase p85 alpha Antibody (3C11) [H00005295-M03] - Analysis of PIK3R1 expression in NIH/3T3 (Cat # L018V1).")
![Western Blot: PI 3-Kinase p85 alpha Antibody (3C11) [H00005295-M03] - Analysis of PIK3R1 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-3C11-Western-Blot-H00005295-M03-img0007.jpg)
![Western Blot: PI 3-Kinase p85 alpha Antibody (3C11) [H00005295-M03] - Analysis of PIK3R1 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-3C11-Western-Blot-H00005295-M03-img0008.jpg)
![Western Blot: PI 3-Kinase p85 alpha Antibody (3C11) [H00005295-M03] - Analysis of PIK3R1 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-3C11-Western-Blot-H00005295-M03-img0009.jpg)
![Sandwich ELISA: PI 3-Kinase p85 alpha Antibody (3C11) [H00005295-M03] - Detection limit for recombinant GST tagged PIK3R1 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-3C11-Sandwich-ELISA-H00005295-M03-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody [NBP1-89731] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89731-img0006.jpg "Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody [NBP1-89731] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody [NBP1-89731] - Staining of human stomach shows strong cytoplasmic positivity in parietal cells.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-89731-img0001.jpg)
![Western Blot: PI 3-Kinase p85 alpha Antibody (M253) [NBP3-23296] - Western blot image of human A431 cells. The blots were probed with mouse monoclonal anti-PI 3-Kinase p85 alpha (C-terminal region) at 1:1000 (lane 1), 1:2000 (lane 2), and 1:4000 (lanes 3).](http://images.novusbio.com/fullsize/nbp3-23296_mouse-pi-3-kinase-p85-alpha-mab-m253-199202312523621.jpg "Western Blot: PI 3-Kinase p85 alpha Antibody (M253) [NBP3-23296] - Western blot image of human A431 cells. The blots were probed with mouse monoclonal anti-PI 3-Kinase p85 alpha (C-terminal region) at 1:1000 (lane 1), 1:2000 (lane 2), and 1:4000 (lanes 3).")
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (M253) [NBP3-23296] - Immunocytochemical labeling of PI 3-Kinase p85 alpha in aldehyde-fixed and NP-40-permeabilized A431 cells. The cells were labeled with mouse monoclonal PI 3-Kinase p85 alpha (C-terminal region) antibody (NBP3-23296), then the antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23296_mouse-pi-3-kinase-p85-alpha-mab-m253-199202318202914.jpg)