PI 3-Kinase p85 alpha Antibody (572106) [Unconjugated]

Western blot shows lysates of A172 human glioblastoma cell line, K562 human chronic myelogenous leukemia cell line, BaF3 mouse pro-B cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat PI 3-Kinase p85a Monoclonal Antibody (Catalog # MAB2998) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PI 3-Kinase p85a at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of A172 human glioblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PI 3‑Kinase p85 alpha at approximately 89 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat PI 3‑Kinase p85 alpha Monoclonal Antibody (Catalog # MAB2998). This experiment was conducted under reducing conditions and using the12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western
• Clone: 572106
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

PI 3-Kinase p85 alpha Antibody (572106) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human PI 3-Kinase p85 alpha
  aa Lys519-Gly644
  Accession # P27986
• Specificity: Detects human, mouse and rat PI 3-Kinase p85 alpha in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PIK3R1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PI 3-Kinase p85 alpha Antibody (572106) [Unconjugated]

• AGM7
• GRB1
• IMD36
• p85
• p85-ALPHA
• Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha
• phosphatidylinositol 3-kinase regulatory subunit alpha
• phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)
• phosphatidylinositol 3-kinase-associated p-85 alpha
• phosphoinositide-3-kinase regulatory subunit alpha
• phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
• PI 3Kinase p85 alpha
• PI 3-Kinase p85 alpha
• PI3K regulatory subunit alpha
• PI3-kinase regulatory subunit alpha
• PI3-kinase subunit p85-alpha
• PIK3R1
• PtdIns-3-kinase regulatory subunit alpha
• PtdIns-3-kinase regulatory subunit p85-alpha

Background

PI 3-Kinases phosphorylate the inositol ring on a variety of membrane-associated phosphatidylinositols. Class I phosphoinositide 3-kinases (PI 3-Kinases) are heterodimeric proteins that consist of a catalytic subunit of 110‑120 kDa and an associated regulatory subunit. p85 alpha is one of the regulatory subunits that associate with the class IA PI 3-Kinases. Composed of one SH3 and two SH2 domains, p85 alpha functions as an adaptor, coupling catalytic p110 to activated receptor tyrosine kinases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PI 3-Kinase p85 alpha Antibody (MAB2998)(5)

Publications using MAB2998

• Futoon Abedrabbu Al-Rawashde, Abdullah Saleh Al-wajeeh, Mansoureh Nazari Vishkaei, Hanan Kamel M. Saad, Muhammad Farid Johan, Wan Rohani Wan Taib et al. Thymoquinone Inhibits JAK/STAT and PI3K/Akt/ mTOR Signaling Pathways in MV4-11 and K562 Myeloid Leukemia Cells Pharmaceuticals (Basel) 9/8/2022 [PMID: 36145344]
• Futoon Abedrabbu Al-Rawashde, Abdullah Saleh Al-wajeeh, Mansoureh Nazari Vishkaei, Hanan Kamel M. Saad, Muhammad Farid Johan, Wan Rohani Wan Taib et al. Thymoquinone Inhibits JAK/STAT and PI3K/Akt/ mTOR Signaling Pathways in MV4-11 and K562 Myeloid Leukemia Cells Pharmaceuticals (Basel) 2022-09-08 [PMID: 36145344] (Western Blot, Human)
• Michael J. Houghton, Asimina Kerimi, Vincent Mouly, Sarka Tumova, Gary Williamson Gut microbiome catabolites as novel modulators of muscle cell glucose metabolism The FASEB Journal 2/1/2019 [PMID: 30183376]
• Michael J. Houghton, Asimina Kerimi, Vincent Mouly, Sarka Tumova, Gary Williamson Gut microbiome catabolites as novel modulators of muscle cell glucose metabolism The FASEB Journal 2019-02-01 [PMID: 30183376] (Capillary Immunoblot, Human)
• Han Y, Wu G, Deng J, Tao J, Guo L, Tian X, Kang J, Zhang X, Yan C Cellular repressor of E1A-stimulated genes inhibits human vascular smooth muscle cell apoptosis via blocking P38/JNK MAP kinase activation. J. Mol. Cell. Cardiol., 2010-01-06;48(6):1225-35. 2010-01-06 [PMID: 20060003] (Western Blot, Human)

Bioinformatics

• Gene Symbol: PIK3R1
• Uniprot:
  • P27986()