PI 3-Kinase p55 gamma Antibody (668619) [Alexa Fluor® 647]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 668619
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

PI 3-Kinase p55 gamma Antibody (668619) [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human p55 γ
  Lys251-Gly378
  Accession # Q92569
• Specificity: Detects human, mouse, and rat PI 3‑Kinase p55 gamma in Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) PIK3R1 (aa 519‑644) and no cross-reactivity with rhPIK3R1 (aa 328-431), rhPIK3R2 (aa 325-428), rhPIK3R2 (aa
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PI 3-Kinase p55 gamma Antibody (668619) [Alexa Fluor® 647]

• DKFZp686P05226
• FLJ41892
• p55
• p55-GAMMA
• p55PIK
• Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma
• phosphatidylinositol 3-kinase regulatory subunit gamma
• phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 3 (p55, gamma)
• phosphoinositide-3-kinase, regulatory subunit 3 (gamma)
• PI 3Kinase p55 gamma
• PI 3-Kinase p55 gamma
• PI3K regulatory subunit gamma
• PI3-kinase regulatory subunit gamma
• PI3-kinase subunit p55-gamma
• PIK3R3
• PtdIns-3-kinase regulatory subunit gamma
• ptdIns-3-kinase regulatory subunit p55-gamma
• regulatory subunit, polypeptide 3 (p55, gamma)

Background

p55 gamma (Protein of 55 kDa, gamma subunit; also PI3-kinase regulatory subunit gamma and p55PIK) is a 55-57 kDa member of the PI3K p85 family of regulatory subunits. It is widely expressed, and serves as a recruitment subunit for the p110 catalytic subunit of PI3 kinase. p55 gamma /p55PIK should not be confused with the 55 kDa alpha splice variant of the p85a gene (75% amino acid [aa] identity). Human p55 gamma is 461 aa in length. It contains a unique 34 aa N-terminus that binds Rb and prevents cycle progression, followed by a Pro-rich region (aa 35-44) and two SH2 domains that bind the p110 catalytic subunit (aa 65-160 and 358-452). Based on mouse, it is likely that alternate start sites at Met8 and Met32 generate 54 and 50 kDa protein products, respectively. There is also a potential for a start site 18 aa upstream of the standard start site, as well as a splice form that shows a deletion of aa 256-314 that may be accompanied by an additional deletion of aa 36-71. Over aa 251-378, human p55 gamma shares 94% aa identity with mouse p55 gamma .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.