HAF007). A specific band was detected for p55 gamma at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HEK293 human embryonic kidney cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with 1 µg/mL of Human/Mouse/Rat PI 3-Kinase p55 gamma Monoclonal Antibody (Catalog # MAB6638) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for p55 gamma at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6638-SP | |||
Immunoblot Buffer Group 1." alt="Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, C2C12 mouse myoblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PI 3-Kinase p55 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5734) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PI 3-Kinase p55 gamma at approximately 55 to 57 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5734-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57974-25ul | |||
![Western Blot: PIK3R3 Antibody [NBP1-32636] - Sample (30ug whole cell lysate)A:A431 7. 5% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PIK3R3-Antibody-Western-Blot-NBP1-32636-img0004.jpg "Western Blot: PIK3R3 Antibody [NBP1-32636] - Sample (30ug whole cell lysate)A:A431 7. 5% SDS PAGE, antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: PIK3R3 Antibody [NBP1-32636] - Paraffin-embedded SAS xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PIK3R3-Antibody-Immunohistochemistry-Paraffin-NBP1-32636-img0002.jpg)
![Western Blot: PIK3R3 Antibody [NBP1-32636] - 30 ug PC-12 whole cell lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/PIK3R3-Antibody-Western-Blot-NBP1-32636-img0003.jpg)
![Western Blot: PIK3R3 Antibody (2F8) [H00008503-M02] - PIK3R3 monoclonal antibody (M02), clone 2F8. Analysis of PIK3R3 expression in human spleen.](http://images.novusbio.com/fullsize/PIK3R3-Antibody-2F8-Western-Blot-H00008503-M02-img0003.jpg "Western Blot: PIK3R3 Antibody (2F8) [H00008503-M02] - PIK3R3 monoclonal antibody (M02), clone 2F8. Analysis of PIK3R3 expression in human spleen.")
![Proximity Ligation Assay: PIK3R3 Antibody (2F8) [H00008503-M02] - Analysis of protein-protein interactions between EGFR and PIK3R3. HeLa cells were stained with anti-EGFR rabbit purified polyclonal 1:1200 and anti-PIK3R3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/PIK3R3-Antibody-2F8-Proximity-Ligation-Assay-H00008503-M02-img0001.jpg)
![Sandwich ELISA: PIK3R3 Antibody (2F8) [H00008503-M02] - Detection limit for recombinant GST tagged PIK3R3 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PIK3R3-Antibody-2F8-Sandwich-ELISA-H00008503-M02-img0002.jpg)
![Western Blot: PI 3-Kinase p55 gamma Antibody (2I10M9) [NBP3-16308] - Western blot analysis of extracts of various cell lines, using PI 3-Kinase p55 gamma Rabbit mAb (NBP3-16308) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/PI-3-Kinase-p55-gamma-Antibody-2I10M9-Western-Blot-NBP3-16308-img0001.jpg "Western Blot: PI 3-Kinase p55 gamma Antibody (2I10M9) [NBP3-16308] - Western blot analysis of extracts of various cell lines, using PI 3-Kinase p55 gamma Rabbit mAb (NBP3-16308) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: PIK3R3 Antibody [NBP2-57974] - Staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.](http://images.novusbio.com/fullsize/PIK3R3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57974-img0001.jpg "Immunocytochemistry/Immunofluorescence: PIK3R3 Antibody [NBP2-57974] - Staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.")
![Western Blot: PIK3R3 Antibody [NBP1-58332] - Transfected 293T cell lysate, concentration 5.0 ug/ml.](http://images.novusbio.com/fullsize/PIK3R3-Antibody-Western-Blot-NBP1-58332-img0002.jpg "Western Blot: PIK3R3 Antibody [NBP1-58332] - Transfected 293T cell lysate, concentration 5.0 ug/ml.")
