PI 3-Kinase p110 gamma Antibody (304103)

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human PI 3-Kinase p110 gamma Monoclonal Antibody (Catalog # MAB2999) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for PI 3-Kinase p110 gamma at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 304103
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

PI 3-Kinase p110 gamma Antibody (304103) Summary

• Immunogen: E. coli-derived recombinant human PI 3-Kinase p110 gamma
  Lys298-Leu467
  Accession # P48736
• Specificity: Detects endogenous human PI 3-Kinase p110 gamma in Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: PIK3CG
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PI 3-Kinase p110 gamma Antibody (304103)

• 1-phosphatidylinositol 3-kinase
• EC 2.7.1
• EC 2.7.1.153
• p110-gamma
• p120-PI3K
• phosphatidylinositol 3 kinase gamma, p110 gamma
• phosphatidylinositol 3-kinase catalytic 110-kD gamma
• phosphatidylinositol 3-kinase, catalytic, gamma polypeptide
• Phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma
• phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma isoform
• phosphoinositide-3-kinase gamma catalytic subunit
• phosphoinositide-3-kinase, catalytic, gamma polypeptide
• PI 3Kinase p110 gamma
• PI 3-Kinase p110 gamma
• PI3CG
• PI3K
• PI3K-gamma
• PI3Kgamma
• PI3K-gamma
• PI3-kinase subunit gamma
• PIK3
• PIK3CG
• PtdIns-3-kinase subunit gamma
• PtdIns-3-kinase subunit p110-gamma

Background

Class I phosphoinositide 3-kinases (PI 3-Kinases) are dual-specific lipid and protein kinases involved in numerous intracellular signaling pathways. Class IA PI 3‑Kinases (p110 alpha , beta  and δ) are typically activated through receptor tyrosine kinase signaling, whereas p110 gamma , the single member of class IB, is activated by seven‑transmembrane G‑protein‑coupled receptors (GPCRs). While p110 alpha and beta are ubiquitiously expressed, expression of p110 gamma is mainly restricted to cells of the hematopoietic system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PI 3-Kinase p110 gamma Antibody (MAB2999)(2)

Publications using MAB2999

• Corey EA, Bobkov Y, Pezier A, Ache BW Phosphoinositide 3-kinase mediated signaling in lobster olfactory receptor neurons. J. Neurochem., 2010-02-02;113(2):341-50. 2010-02-02 [PMID: 20132480] (ICC, Western Blot, Lobster)
• Matsuda N, Yamamoto S, Yokoo H, Tobe K, Hattori Y Nuclear factor-kappaB decoy oligodeoxynucleotides ameliorate impaired glucose tolerance and insulin resistance in mice with cecal ligation and puncture-induced sepsis. Crit. Care Med., 2009-10-01;37(10):2791-9. 2009-10-01 [PMID: 19707125] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: PIK3CG
• Uniprot:
  • P48736()