Phospholipid Scramblase 1/PLSCR1 Antibody

Western Blot: Phospholipid Scramblase 1/PLSCR1 Antibody [NBP1-32588] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Phospholipid scramblase 1 antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Phospholipid Scramblase 1/PLSCR1 Antibody [NBP1-32588] - A431 cells were fixed in ice-cold MeOH for 5 min. Green: PLSCR1 protein stained by Scramblase1 antibody [N1C3] diluted at 1:5000. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: Phospholipid Scramblase 1/PLSCR1 Antibody [NBP1-32588] - MDAMB468 xenograft , using phospholipid scramblase 1 antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: Phospholipid Scramblase 1/PLSCR1 Antibody [NBP1-32588] - Phospholipid scramblase is required for phosphatidylserine and Akt relocalization.CaSki cells were mock-infected or infected with HSV-2(G) and 15 minutes, 30 minutes, 1 hour and 4 hours post-infection, cell lysates were harvested. Lysates were incubated with a goat anti-PLSCR1 antibody and immune complexes precipitated with protein A-agarose and analyzed by Western blotting with a mouse anti-phosphotyrosine (PY20) or mouse anti-PLSCR1 mAb. The blot is representative of results obtained in 2 independent experiments. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1006766) licensed under a CC-BY license.

• Reactivity: Hu, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Phospholipid Scramblase 1/PLSCR1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Phospholipid Scramblase 1/PLSCR1. The exact sequence is proprietary.
• Localization: Membrane, Single-pass type II membrane protein
• Predicted Species: Rhesus Macaque (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PLSCR1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS pH7, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Phospholipid Scramblase 1/PLSCR1 Antibody

• Erythrocyte phospholipid scramblase
• MMTRA1B
• MMTRA1BCa(2+)-dependent phospholipid scramblase 1
• Phospholipid Scramblase 1
• PL scramblase 1
• PLSCR1

Background

Phospholipid scramblase 1 (PLSCR1) is a member of the PLSCR protein family. These proteins are ATP-independent lipid translocators localized to the plasma membrane and is involved in a calcium dependent, nonspecific and rapid redistribution (scrambling) of phospholipids when a perturbation of plasma membrane asymmetry is needed for the cell (e.g. following cell activation, injury, or apoptosis). The expression of PLSCR1 is found in a variety of tissue and can be stress-induced. Upon stimulation (e.g. interferon stimulation), a portion of newly synthesized PLSCR1 is imported into the nucleus (instead of inserted into the plasma membrane) and interacts with DNA, consistent with a role in gene regulation. PLSCR1 may be closely associated with the pathophysiology underlying infection and inflammation, which manifest marked changes in energy consumption and lipid metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Phospholipid Scramblase 1/PLSCR1 Antibody (NBP1-32588)(2)

Publications using NBP1-32588

• Cheshenko N, Bonanno JB, Hoffmann HH et al. Cell-impermeable staurosporine analog targets extracellular kinases to inhibit HSV and SARS-CoV-2 Communications biology 2022-10-16 [PMID: 36245045] (Biacore Binding Assay, Human)
• Cheshenko N, Pierce C, Herold BC. Herpes simplex viruses activate phospholipid scramblase to redistribute phosphatidylserines and Akt to the outer leaflet of the plasma membrane and promote viral entry PLoS Pathog. 2018-01-01 [PMID: 29293671] (Human)

Bioinformatics

• Gene Symbol: PLSCR1