Phospho-Akt (S473) Pan Specific DuoSet IC ELISA, 2 Plate

• Reactivity: Hu, Mu, Rt
• Applications: ELISA
• Conjugate: Biotin

Phospho-Akt (S473) Pan Specific DuoSet IC ELISA, 2 Plate Summary

• Modification: p Ser473
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Phospho-Akt (S473) Pan Specific DuoSet IC ELISA, 2 Plate

• RAC-ALPHA
• AKT serine/threonine kinase 1
• Akt
• PKB
• PKB-ALPHA
• PRKBA
• RAC

Background

Akt1, Akt2, and Akt3 make up a family of serine/threonine kinases, often activated downstream of growth factor receptors and PI 3-Kinase. Each Akt family member contains an N-terminal pleckstrin homology (PH) domain, a central kinase domain, and a C-terminal regulatory domain. They have putative roles in cell proliferation and survival, and Akt perturbations are thought to play an important role in tumorigenesis.

Publications for AKT (DYC887B-2)(18)

Publications using DYC887B-2

• Kadry, MO; miR-122-IGF-1R signaling allied through the dysregulated lncRNA MALAT-1 expression in gastric carcinoma Toxicology reports 2024-06-01 [PMID: 38544957] (Rat)
• Gong, GQ;Bilanges, B;Allsop, B;Masson, GR;Roberton, V;Askwith, T;Oxenford, S;Madsen, RR;Conduit, SE;Bellini, D;Fitzek, M;Collier, M;Najam, O;He, Z;Wahab, B;McLaughlin, SH;Chan, AWE;Feierberg, I;Madin, A;Morelli, D;Bhamra, A;Vinciauskaite, V;Anderson, KE;Surinova, S;Pinotsis, N;Lopez-Guadamillas, E;Wilcox, M;Hooper, A;Patel, C;Whitehead, MA;Bunney, TD;Stephens, LR;Hawkins, PT;Katan, M;Yellon, DM;Davidson, SM;Smith, DM;Phillips, JB;Angell, R;Williams, RL;Vanhaesebroeck, B; A small-molecule PI3K? activator for cardioprotection and neuroregeneration Nature 2023-06-01 [PMID: 37225977] (Human)
• N Page, M Wappett, CR O'Dowd, M O'Rourke, G Gavory, L Zhang, JSS Rountree, L Jordan, O Barker, H Gibson, C Boyd, S Feutren-Bu, E McLean, G Trevitt, T Harrison Identification and development of a subtype-selective allosteric AKT inhibitor suitable for clinical development Scientific Reports, 2022-09-20;12(1):15715. 2022-09-20 [PMID: 36127435] (Human)
• DA Olsen, CEB Thomsen, RF Andersen, JS Madsen, A Jakobsen, I Brandslund Decreased concentrations of intracellular signaling proteins in colon cancer patients with BRAF mutations Sci Rep, 2020-11-18;10(1):20113. 2020-11-18 [PMID: 33208845] (Human)
• D Biswas, KT Dao, A Mercer, AM Cowie, L Duffley, Y El Hiani, PC Kienesberg, T Pulinilkun Branched-chain ketoacid overload inhibits insulin action in the muscle J. Biol. Chem., 2020-09-02;0(0):. 2020-09-02 [PMID: 32878988] (Mouse)
• Z Julier, R Karami, B Nayer, YZ Lu, AJ Park, K Maruyama, GA Kuhn, R Müller, S Akira, MM Martino Enhancing the regenerative effectiveness of growth factors by local inhibition of interleukin-1 receptor signaling Sci Adv, 2020;6(24):eaba7602. 2020 [PMID: 32582857] (Mouse)
• Z Julier, R Karami, B Nayer, YZ Lu, AJ Park, K Maruyama, GA Kuhn, R Müller, S Akira, MM Martino Enhancing the regenerative effectiveness of growth factors by local inhibition of interleukin-1 receptor signaling Sci Adv, 2020-06-12;6(24):eaba7602. 2020-06-12 [PMID: 32582857] (Mouse)
• A Tutusaus, E de Gregori, B Cucarull, H Cristóbal, C Aresté, I Graupera, M Coll, A Colell, G Gausdal, JB Lorens, P García de, A Morales, M Marí A FUNCTIONAL ROLE OF GAS6/TAM IN NON-ALCOHOLIC STEATOHEPATITIS PROGRESSION IMPLICATES AXL AS THERAPEUTIC TARGET Cell Mol Gastroenterol Hepatol, 2019-11-02;0(0):. 2019-11-02 [PMID: 31689560] (Human)
• G Zhou, X Han, Z Wu, Q Shi, X Bao Rosiglitazone accelerates wound healing by improving endothelial precursor cell function and angiogenesis in db/db mice PeerJ, 2019-10-17;7(0):e7815. 2019-10-17 [PMID: 31637120] (Mouse)
• B Marcus-Sam, CC Krieger, A Boutin, G Kahaly, S Neumann, MC Gershengor Evidence that Graves' Ophthalmopathy Immunoglobulins Do Not Directly Activate IGF-1 Receptors Thyroid, 2018-04-30;0(0):. 2018-04-30 [PMID: 29631510] (Human)
• Neurotensin stimulates sortilin and mTOR in human microglia inhibitable by methoxyluteolin, a potential therapeutic target for autism Proc Natl Acad Sci USA, 2016-09-23;0(0):. 2016-09-23 [PMID: 27663735] (Human)
• FEDERICA RUFFINI, STEFANIA D’ATRI, PEDRO M. LACAL Neuropilin-1 expression promotes invasiveness of melanoma cells through vascular endothelial growth factor receptor-2-dependent and -independent mechanisms International Journal of Oncology 2013-07-01 [PMID: 23685409] (Human)
• Ray , Rupa, de Ridder , Gustaaf, Eu , Jerry P, Paton , Adrienne, Paton , James C, Pizzo , Salvator The Escherichia coli subtilase cytotoxin A subunit specifically cleaves cell-surface GRP78 protein and abolishes COOH-terminal-dependent signaling. J Biol Chem, 2012-07-31;287(39):32755-69. 2012-07-31 [PMID: 22851173] (Human)
• Zhang Y, Gonzalez RM, Zangar RC Protein secretion in human mammary epithelial cells following HER1 receptor activation: influence of HER2 and HER3 expression. BMC Cancer, 2011-02-14;11(0):69. 2011-02-14 [PMID: 21320340] (Human)
• Yeh CC, Li H, Malhotra D, Turcato S, Nicholas S, Tu R, Zhu BQ, Cha J, Swigart PM, Myagmar BE, Baker AJ, Simpson PC, Mann MJ Distinctive ERK and p38 signaling in remote and infarcted myocardium during post-MI remodeling in the mouse. J. Cell. Biochem., 2010-04-15;109(6):1185-91. 2010-04-15 [PMID: 20186881] (Mouse)
• Hsieh CH, Nickel EA, Chen J, Schwacha MG, Choudhry MA, Bland KI, Chaudry IH Mechanism of the salutary effects of estrogen on kupffer cell phagocytic capacity following trauma-hemorrhage: pivotal role of Akt activation. J. Immunol., 2009-04-01;182(7):4406-14. 2009-04-01 [PMID: 19299741] (Mouse)
• Struyf S, Noppen S, Loos T, Mortier A, Gouwy M, Verbeke H, Huskens D, Luangsay S, Parmentier M, Geboes K, Schols D, Van Damme J, Proost P Citrullination of CXCL12 differentially reduces CXCR4 and CXCR7 binding with loss of inflammatory and anti-HIV-1 activity via CXCR4. J. Immunol., 2009-01-01;182(1):666-74. 2009-01-01 [PMID: 19109200] (Human)
• Loos T, Mortier A, Gouwy M, Ronsse I, Put W, Lenaerts JP, Van Damme J, Proost P Citrullination of CXCL10 and CXCL11 by peptidylarginine deiminase: a naturally occurring posttranslational modification of chemokines and new dimension of immunoregulation. Blood, 2008-07-21;112(7):2648-56. 2008-07-21 [PMID: 18645041] (Hamster)