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Recombinant Human PGM1 Protein

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SDS-Page: PGM1 Recombinant Protein [NBP2-52160] - 15% SDS Page (3 ug)
SDS-Page: PGM1 Recombinant Protein [NBP2-52160] - 15% SDS Page (3 ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

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Recombinant Human PGM1 Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-562 of Human PGM1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMVKIVTV KTQAYQDQKP GTSGLRKRVK VFQSSANYAE NFIQSIISTV EPAQRQEATL VVGGDGRFYM KEAIQLIARI AAANGIGRLV IGQNGILSTP AVSCIIRKIK AIGGIILTAS HNPGGPNGDF GIKFNISNGG PAPEAITDKI FQISKTIEEY AVCPDLKVDL GVLGKQQFDL ENKFKPFTVE IVDSVEAYAT MLRSIFDFSA LKELLSGPNR LKIRIDAMHG VVGPYVKKIL CEELGAPANS AVNCVPLEDF GGHHPDPNLT YAADLVETMK SGEHDFGAAF DGDGDRNMIL GKHGFFVNPS DSVAVIAANI FSIPYFQQTG VRGFARSMPT SGALDRVASA TKIALYETPT GWKFFGNLMD ASKLSLCGEE SFGTGSDHIR EKDGLWAVLA WLSILATRKQ SVEDILKDHW QKYGRNFFTR YDYEEVEAEG ANKMMKDLEA LMFDRSFVGK QFSANDKVYT VEKADNFEYS DPVDGSISRN QGLRLIFTDG SRIVFRLSGT GSAGATIRLY IDSYEKDVAK INQDPQVMLA PLISIALKVS QLQERTGRTA PTVIT
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
PGM1
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
63.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human PGM1 Protein

  • EC 5.4.2
  • EC 5.4.2.2
  • Glucose phosphomutase 1
  • GSD14
  • PGM 1
  • phosphoglucomutase 1
  • phosphoglucomutase-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Flow (Intracellular): Complement C3 Antibody (11H9) [NB200-540] - An intracellular stain was performed on RAW 246.7 cells with Complement C3 (11H9-3-2) antibody NB200-540 (blue) and a matched isotype control NBP2-31382 (orange). Cells were either treated with 3uM Monensin for 3 hours to block the secretion of Complement C3 (B) or grown in normal media (A). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).
Complement C3 Antibody (11H9)
NB200-540
Western Blot: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - Detection of GLO-1 in human cell lines. Lane 1,2 and 3: MG-63 osteosarcoma cell line (positive control), 402-91 liposarcoma cell line and 402-91 liposarcoma cell line resistant to trabectedine. This image was submitted via customer Review.
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Bioinformatics

Gene Symbol PGM1