PGLYRP4/PGRP-I beta Antibody (186C426)

Images

 
Western Blot: PGLYRP4/PGRP-I beta Antibody (186C426) [NB100-56721] - Analysis in cell lysates from human brain using a dilution of 2 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
186C426
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

PGLYRP4/PGRP-I beta Antibody (186C426) Summary

Immunogen
This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 95-110 of human PGRP-1 beta.
Specificity
PGRP-1b (186C426)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PGLYRP4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
Flow Cytometry (Cell Surface): See Uehara (2005) et al. Immunocytochemistry/Immunofluorescence: See Uehara (2005) et al.
Positive Control
Brain Lysate (NB820-59177)
Publications
Read Publications using
NB100-56721 in the following applications:

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.0.5% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for PGLYRP4/PGRP-I beta Antibody (186C426)

  • peptidoglycan recognition protein 4
  • peptidoglycan recognition protein I beta
  • Pglyrp-1 beta
  • PGLYRP4
  • PGRPI beta
  • PGRP-I beta
  • PGRP-I-beta

Background

The primary immune recognition is based on structures common among invading pathogens. Bacterial surface molecules, such as lipopolysaccharide (LPS) and peptidoglycan (PGN), are known to elicit immune reactions ranging from cytokine release to fever. Recently, a family of proteins called peptidoglycan recognition protein (PGRP) has been identified in mouse and human that binds to peptidoglycans expressed on Gram-positive bacteria. Peptidoglycan (PGN) is an essential cell wall component of virtually all bacteria and, thus, it is an excellent target for recognition by the eukaryotic innate immune system. The PGRPs (PGRP-L, PGRP-S, PGRP-I alpha, and PGRP-I beta) define a new family of human pattern recognition molecules. PGRP-L is primarily expressed in the liver. Although liver is not considered a primary immune organ, liver participates in host defenses by producing acute phase proteins (by hepatocytes) in response to infections and by clearing microorganisms from blood.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for PGLYRP4/PGRP-I beta Antibody (NB100-56721)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Flow-CS, Flow-IC, ICC/IF.


Filter By Application
Flow-CS
(3)
Flow-IC
(1)
ICC/IF
(3)
All Applications
Filter By Species
Human
(3)
All Species
Showing Publications 1 - 6 of 6.
Publications using NB100-56721 Applications Species
Hua X, Yuan X, Li Z et al. A Novel Innate Response of Human Corneal Epithelium to Heat-killed Candida albicans by Producing Peptidoglycan Recognition Proteins PLoS ONE. 2015 Jun 04 [PMID: 26039076] (ICC/IF, Human)

Details:
PGLYRP4/PGRP-I beta antibody (clone 186C426; Imgenex IMG-414) was used for ICC-IF staining on human corneal epithelial cells /HCECs that were incubated in the absence or presence of isotype IgG (10ug/ml), dectin-1 neutralizing Ab (10ug/ml), BAY11-7082 (10uM) or NF-kB activation inhibitor quinazoline (NF-kB-I, 10uM) for 1 h followed by 48 hours incubation of HKCA/heat-killed Candida albicans (10^6 cells/ml). The immunoassay implicated 10 minutes RT fixation with 2% PFA, 10 minutes RT permeabilization with PBS-0.2% Triton X-100, detection of primary using Alexa-Fluor 488 conjugated secondary antibody (Fig 4A).
ICC/IF Human
Ma P, Wang Z, Pflugfelder SC, Li DQ. Toll-like receptors mediate induction of peptidoglycan recognition proteins in human corneal epithelial cells. Exp Eye Res. 2010 Jan [PMID: 19799901] (ICC/IF, Human)

Details:
PGRP-1alpha (IMG-391) & PGRP-1beta (IMG-414). IF/ICC: Human corneoscleral tissue & primary cultured human corneal epithelial cells, Fig 1.
ICC/IF Human
Uehara A, Takada H. Synergism between TLRs and NOD1/2 in oral epithelial cells. J Dent Res. 2008 Jul [PMID: 18573991] (Flow-CS)

Details:
flow (cell surface): PGRP-1alpha (IMG-391), PGRP-1beta (IMG-414), PGRP-S (IMG-393). Oral epithelial HSC-2 cell line stimulated with Fk156 or muramyldipeptide (MDP) plus FSL-1 and lipid A, Fig 1.
Flow-CS
Uehara A, Fujimoto Y, Fukase K, Takada H. Various human epithelial cells express functional Toll-like receptors, NOD1 and NOD2 to produce anti-microbial peptides, but not proinflammatory cytokines. Mol Immunol. 2007 May [PMID: 17403538] (Flow-CS, Human)

Details:
The following products were used in flow (cell surface): PGRP-1alpha (IMG-391), PGRP-1beta (IMG-414), PGRP-S (IMG-393). Human oral epithelial (HSC-2, HSC-3, SAS, & HO-1-u-1), human pharyngeal epithelial (HEp-2), human esophageal epithelial (TE-1), human b
Flow-CS Human
Uehara A, Fujimoto Y, Kawasaki A et al. Meso-diaminopimelic acid and meso-lanthionine, amino acids specific to bacterial peptidoglycans, activate human epithelial cells through NOD1. J Immunol. 2006 Aug 1 [PMID: 16849490] (Flow-IC)

Details:
flow (Intracellular): PGRP-1alpha (IMG-391) & PGRP-1beta (IMG-414); Human oral epithelial (HSC-2) cell line, Fig 2.
Flow-IC
Uehara A, Sugawara Y, Kurata S et al. Chemically synthesized pathogen-associated molecular patterns increase the expression of peptidoglycan recognition proteins via toll-like receptors, NOD1 and NOD2 in human oral epithelial cells. Cell Microbiol. 2005 May [PMID: 15839897] (Flow-CS, ICC/IF)

Details:
Flow (cell surface): PGRP-1alpha (IMG-391), PGRP-1beta (IMG-414), and PGRP-S (IMG-393) were used in human oral epithelial (HSC-2) cell lines, Fig 3. IF/ICC: HSC-2 cells stimulated with or without lipid A, muramyldipeptide (MDP), gama-D-glutamyl-meso-DAP (iE-DAP), or IFN gamma then stained with PGRP-1beta (IMG-414), Fig 4.
Flow-CS, ICC/IF

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PGLYRP4/PGRP-I beta Antibody (NB100-56721) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56721AF405
Alexa Fluor 488 Labeled NB100-56721AF488
Alexa Fluor 647 Labeled NB100-56721AF647
Alexa Fluor 700 Labeled NB100-56721AF700
Biotin Labeled NB100-56721B
DyLight 350 Labeled NB100-56721UV
DyLight 405 Labeled NB100-56721V
DyLight 488 Labeled NB100-56721G
DyLight 550 Labeled NB100-56721R
DyLight 650 Labeled NB100-56721C
DyLight 680 Labeled NB100-56721FR
DyLight 755 Labeled NB100-56721IR
HRP Labeled NB100-56721H

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Bioinformatics

Gene Symbol PGLYRP4
Entrez
Uniprot