PGD2 Synthase/PTGDS Antibody (997303) [Unconjugated]

Western blot shows lysates of human heart tissue and rat brain tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Rat PGD2 Synthase/PTGDS Monoclonal Antibody (Catalog # MAB10099) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PGD2 Synthase/PTGDS at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

PGD2 Synthase/PTGDS was detected in immersion fixed paraffin-embedded sections of human brain (caudate nucleus) using Mouse Anti-Human/Rat PGD2 Synthase/PTGDS Monoclonal Antibody (Catalog # MAB10099) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu, Rt
• Applications: WB, IHC
• Clone: 997303
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

PGD2 Synthase/PTGDS Antibody (997303) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human PGD2 Synthase/PTGDS
  Met1-Gln190
  Accession # P41222
• Specificity: Detects human and rat PGD2 Synthase/PTGDS in direct ELISA and Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-25 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PGD2 Synthase/PTGDS Antibody (997303) [Unconjugated]

• Beta-trace protein
• Cerebrin-28
• EC 5.3.99.2
• Glutathione-independent PGD synthase
• glutathione-independent PGD synthetase
• lipocalin-type prostaglandin D synthase
• Lipocalin-type prostaglandin-D synthase
• L-PGDS
• PDS
• PGD2 Synthase
• PGD2
• PGDS2
• PGDSLPGDS
• PH2DISO
• prostaglandin D synthase
• prostaglandin D2 synthase (21kD, brain)
• prostaglandin D2 synthase 21kDa (brain)
• Prostaglandin-D2 synthase
• prostaglandin-H2 D-isomerase
• PTGDS

Background

Prostaglandin-H2 D-isomerase or PGD2 synthase (PTGDS) is an enzyme that catalyzes the conversion of Prostaglandin H2 (PGH2) to Prostaglandin D2 (PGD2), a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Prostaglandin D2 synthase is used clinically as a diagnostic marker for Cerebrospinal fluid rhinorrhea, important in the assessment of head trauma severity. It has been also proposed that the urinary and serum levels pf PTGDS may be also a marker of renal damage in diabetes mellitus and hypertension.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P41222()