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PGD Antibody (OTI2A5)

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Western Blot: PGD Antibody (OTI2A5) [NBP2-45880] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGD.
Western Blot: PGD Antibody (OTI2A5) [NBP2-45880] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGD.
Immunohistochemistry-Paraffin: PGD Antibody (OTI2A5) [NBP2-45880] - Human thyroid tissue using anti-PGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.
Immunohistochemistry-Paraffin: PGD Antibody (OTI2A5) [NBP2-45880] - Human pancreas tissue within the normal limits using anti-PGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, ...read more
Immunohistochemistry-Paraffin: PGD Antibody (OTI2A5) [NBP2-45880] - Carcinoma of Human prostate tissue using anti-PGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees ...read more
Immunohistochemistry-Paraffin: PGD Antibody (OTI2A5) [NBP2-45880] - Human prostate tissue within the normal limits using anti-PGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, ...read more
Immunohistochemistry-Paraffin: PGD Antibody (OTI2A5) [NBP2-45880] - Human ovary tissue using anti-PGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.
Immunohistochemistry-Paraffin: PGD Antibody (OTI2A5) [NBP2-45880] - Human breast tissue within the normal limits using anti-PGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, ...read more
Immunohistochemistry-Paraffin: PGD Antibody (OTI2A5) [NBP2-45880] - Human Kidney tissue within the normal limits using anti-PGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, ...read more
Immunohistochemistry-Paraffin: PGD Antibody (OTI2A5) [NBP2-45880] - Human endometrium tissue using anti-PGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for ...read more
Immunofluorescence: PGD Antibody (OTI2A5) [NBP2-45880] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY PGD.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, ICC/IF
Clone
OTI2A5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-73361AF350
Alexa Fluor 405
NBP2-73361AF405
Alexa Fluor 488
NBP2-73361AF488
Alexa Fluor 532
NBP2-73361AF532
Alexa Fluor 594
NBP2-73361AF594
Alexa Fluor 647
NBP2-73361AF647
Alexa Fluor 700
NBP2-73361AF700
Alexa Fluor 750
NBP2-73361AF750
Biotin
NBP2-73361B
DyLight 350
NBP2-73361UV
DyLight 405
NBP2-73361V
DyLight 488
NBP2-73361G
DyLight 550
NBP2-73361R
DyLight 594
NBP2-73361DL594
DyLight 650
NBP2-73361C
DyLight 680
NBP2-73361FR
DyLight 755
NBP2-73361IR
FITC
NBP2-73361F
HRP
NBP2-73361H
Janelia Fluor 549
NBP2-73361JF549
Janelia Fluor 646
NBP2-73361JF646
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-73361
100% Guarantee on every product

PGD Antibody (OTI2A5) Summary

Immunogen
Full length human recombinant protein of human PGD(NP_002622) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PGD
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Immunofluorescence 1:100
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Affinity purified

Alternate Names for PGD Antibody (OTI2A5)

  • 6PGD
  • EC 1.1.1.44,6-phosphogluconate dehydrogenase, decarboxylating
  • PGD
  • PGDH
  • phosphogluconate dehydrogenase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] - Immunoblot analyses of protein expression for Nrf2 and antioxidant enzymes. Representative immunoblots of cytosolic extracts from the hearts of young and old mice under basal conditions and following EES. Protein blots were probed with anti-HO1, NQO1, GCLM, GCLC, Catalase, SOD1, SOD2, GSR, G6PD, GPX1 and GAPDH. Individual lanes indicate a single animal. Densitometry analysis of respective protein signals was performed using Image-J and expressed as relative intensity units calculated as mean values of young and old, *p<0.05. Individual lanes indicate each animal (n?=?6). #p<0.05-between basal and EES. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0045697), licensed under a CC-BY licence.
Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] - Detection of Human and Mouse G6PD by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G6PD antibody NB100-236 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] - Detection of mouse G6PD by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-G6PD antibody NB100-236 (lot 2) used for IP at 6 ug per reaction. G6PD was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated G6PD, NB100-236 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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Immunocytochemistry/Immunofluorescence: Glucose 6 phosphate isomerase Antibody (2D2) [NBP2-02541] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Glucose 6 phosphate isomerase.
Immunohistochemistry-Paraffin: Glucose 6 phosphate isomerase Antibody (2D2) [NBP2-02541] - Staining of paraffin-embedded Human prostate tissue using anti-Glucose 6 phosphate isomerasemouse monoclonal antibody.
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Applications: WB, ICC/IF, IHC, IHC-P
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Western Blot: ME1 Antibody [NBP1-32398] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: ME1 Antibody [NBP1-32398] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: ME1 Antibody [NBP1-32398] - Paraffin-embedded human ovarian carcinoma. ME1 antibody dilution: 1:500.
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Western Blot: ME3 Antibody [NBP1-85871] - Analysis in human cell line CAPAN-2.
Immunohistochemistry-Paraffin: ME3 Antibody [NBP1-85871] - Staining of human tonsil shows very weak granular cytoplasmic positivity in germinal center cells.
Immunohistochemistry-Paraffin: ME3 Antibody [NBP1-85871] - Staining of human endometrium shows moderate granular cytoplasmic positivity.
Species: Hu
Applications: WB, IHC, IHC-P
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NBP1-89559
Western Blot: ME2 Antibody [NBP1-89559] - Analysis using Anti-ME2 antibody NBP1-89559 (A) shows similar pattern to independent antibody NBP1-82434 (B).
Immunohistochemistry-Paraffin: ME2 Antibody [NBP1-89559] - Staining of human colon.
Western Blot: ME2 Antibody [NBP1-89559] - Analysis in human cell line Daudi.
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Western Blot: PTGDR Antibody [H00005729-B01P] - Analysis of PTGDR expression in transfected 293T cell line by PTGDR polyclonal antibody. Lane 1: PTGDR transfected lysate(39.6 KDa). Lane 2: Non-transfected lysate.
Flow Cytometry: PTGDR Antibody [H00005729-B01P] - Analysis of negative control 293 cells (Black) and PTGDR expressing 293 cells (Green) using PTGDR purified mouse polyclonal antibody.
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NBP2-03621
Western Blot: UBL4A Antibody (1D4) [NBP2-03621] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBL4A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBL4A.
Immunocytochemistry/Immunofluorescence: UBL4A Antibody (1D4) [NBP2-03621] Staining of COS7 cells transiently transfected by pCMV6-ENTRY UBL4A.
Immunohistochemistry-Paraffin: UBL4A Antibody (1D4) [NBP2-03621] - Staining of paraffin-embedded Human liver tissue using anti-UBL4A mouse monoclonal antibody.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
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Western Blot: PGD2 Synthase/PTGDS Antibody [NBP1-79280] - Titration: 1 ug/ml Positive Control: HepG2 cell lysate.
Immunohistochemistry-Paraffin: PGD2 Synthase/PTGDS Antibody [NBP1-79280] - Staining of mouse brain. Image provided by Allison Brager - Department of Neurobiology, Morehouse School of Medicine.
Immunohistochemistry-Paraffin: PGD2 Synthase/PTGDS Antibody [NBP1-79280] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.
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Immunohistochemistry-Paraffin: Glutathione Reductase Antibody [NBP2-16684] - Paraffin-embedded rat brain. Glutathione reductase antibody [N2C2], Internal diluted at 1:500.
Western Blot: Glutathione Reductase Antibody [NBP2-16684] - Sample (30 ug of whole cell lysate) A: HCT116 7. 5% SDS PAGE gel, diluted at 1:1000.
Species: Hu, Mu, Rt
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Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-689] - Formalin fixed paraffin embedded colon carcinoma stained with COX-2 antibody (NB100-689).
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Western Blot: ESD Antibody (J0-8) [NBP2-66825] - Analysis on different cell lysates using anti-ESD Mouse mAb. Positive control: Lane 1: K562 Lane 2: Jurkat
Immunocytochemistry/Immunofluorescence: ESD Antibody (J0-8) [NBP2-66825] - Staining of SW480 cells using anti-ESD Mouse mAb.
Immunohistochemistry-Paraffin: ESD Antibody (J0-8) [NBP2-66825] - Analysis of paraffin-embedded mouse embryo tissue using anti-ESD Mouse mAb.
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Immunocytochemistry/Immunofluorescence: DIO2 Antibody [NBP1-00178] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton.Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunohistochemistry-Frozen: DIO2 Antibody [NBP1-00178] - IHC analysis of DIO2 in cryo-sectioned Mouse Hippocampus using NBP1-00178 at 2ug/ml. Microwaved antigen retrieval with citrate buffer pH 4.5, HRP-staining.
Flow Cytometry: DIO2 Antibody [NBP1-00178] - Analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5%Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody
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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-73361AF350
Alexa Fluor 405 NBP2-73361AF405
Alexa Fluor 488 NBP2-73361AF488
Alexa Fluor 532 NBP2-73361AF532
Alexa Fluor 594 NBP2-73361AF594
Alexa Fluor 647 NBP2-73361AF647
Alexa Fluor 700 NBP2-73361AF700
Alexa Fluor 750 NBP2-73361AF750
Biotin NBP2-73361B
DyLight 350 NBP2-73361UV
DyLight 405 NBP2-73361V
DyLight 488 NBP2-73361G
DyLight 550 NBP2-73361R
DyLight 594 NBP2-73361DL594
DyLight 650 NBP2-73361C
DyLight 680 NBP2-73361FR
DyLight 755 NBP2-73361IR
FITC NBP2-73361F
HRP NBP2-73361H
Janelia Fluor 549 NBP2-73361JF549
Janelia Fluor 646 NBP2-73361JF646
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol PGD