Recombinant Human PGAM1 Protein

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Recombinant Human PGAM1 Protein [NBP1-48341]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human PGAM1 Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-254 of Human PGAM1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAAYKLVLIR HGESAWNLEN RFSGWYDADL SPAGHEEAKR GGQALRDAGY EFDICFTSVQ KRAIRTLWTV LDAIDQMWLP VVRTWRLNER HYGGLTGLNK AETAAKHGEA QVKIWRRSYD VPPPPMEPDH PFYSNISKDR RYADLTEDQL PSCESLKDTI ARALPFWNEE IVPQIKEGKR VLIAAHGNSL RGIVKHLEGL SEEAIMELNL PTGIPIVYEL DKNLKPIKPM QFLGDEETVR KAMEAVAAQG KAKK

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
PGAM1
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
30.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 1mM DTT
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human PGAM1 Protein

  • BPG-dependent PGAM 1
  • EC 3.1.3.13
  • EC 5.4.2.1
  • EC 5.4.2.4
  • PGAMA
  • PGAM-Bphosphoglycerate mutase 1
  • phosphoglycerate mutase 1 (brain)
  • phosphoglycerate mutase A, nonmuscle form
  • Phosphoglycerate mutase isozyme B

Background

PGAM1 belongs to the phosphoglycerate mutase family. This protein is important components of glucose and 2,3-BPGA (2,3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). Mutations in this protein cause muscle phosphoglycerate mutase eficiency, also known as glycogen storage disease X. Recombinant human PGAM protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol PGAM1