Human PGAM1 ELISA Kit (Colorimetric)

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ELISA: Human PGAM1 ELISA Kit (Colorimetric) [NBP2-81247] - Samples were spiked with high concentrations of Human PGAM1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range ...read more
ELISA: Human PGAM1 ELISA Kit (Colorimetric) [NBP2-81247] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
0.78 - 50 ng/mL
Sensitivity
0.47 ng/mL

Order Details

Human PGAM1 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human PGAM1 in samples. No significant cross-reactivity or interference between Human PGAM1 and analogues was observed.
Standard Curve Range
0.78 - 50 ng/mL
Sensitivity
0.47 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.2%
Intra-Assay
CV% < 4.91%
Spike Recovery
85-105%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
PGAM1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human PGAM1 ELISA Kit (Colorimetric)

  • BPG-dependent PGAM 1
  • EC 3.1.3.13
  • EC 5.4.2.1
  • EC 5.4.2.4
  • PGAMA
  • PGAM-Bphosphoglycerate mutase 1
  • phosphoglycerate mutase 1 (brain)
  • phosphoglycerate mutase A, nonmuscle form
  • Phosphoglycerate mutase isozyme B

Background

PGAM is an enzyme that catalyzes 3 reactions; the isomerization of 2-PGA to 3-PGA with 2,3-DPG as a primer, the synthesis of 2,3-DPG from 1,3-DPG with 3-PGA as a primer and the degradation of 2,3-DPG to 3-PGA. There are two different isoforms of PGAM in mammals, a muscle and brain isoform. PGAM has also been linked to non-spherocytic anemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol PGAM1