Human PGAM1 ELISA Kit (Colorimetric) Summary
Specificity |
This kit recognizes Human PGAM1 in samples. No significant cross-reactivity or interference between Human PGAM1 and analogues was observed. |
Standard Curve Range |
0.78 - 50 ng/mL |
Sensitivity |
0.47 ng/mL |
Assay Type |
Sandwich-ELISA |
Inter-Assay |
CV% < 5.2% |
Intra-Assay |
CV% < 4.91% |
Spike Recovery |
85-105% |
Sample Volume |
100 ul |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
PGAM1 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
|
Alternate Names for Human PGAM1 ELISA Kit (Colorimetric)
Background
PGAM is an enzyme that catalyzes 3 reactions; the isomerization of 2-PGA to 3-PGA with 2,3-DPG as a primer, the synthesis of 2,3-DPG from 1,3-DPG with 3-PGA as a primer and the degradation of 2,3-DPG to 3-PGA. There are two different isoforms of PGAM in mammals, a muscle and brain isoform. PGAM has also been linked to non-spherocytic anemia.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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