PGAM1 Antibody (2G1-A6) - Azide and BSA Free

Western Blot: PGAM1 Antibody (2G1-A6) [H00005223-M01] - Analysis of PGAM1 expression in transfected 293T cell line by PGAM1 monoclonal antibody (M01), clone 2G1-A6.Lane 1: PGAM1 transfected lysate (Predicted MW: 28.8 KDa).Lane 2: Non-transfected lysate.

Western Blot: PGAM1 Antibody (2G1-A6) [H00005223-M01] - PGAM1 monoclonal antibody (M01), clone 2G1-A6 Analysis of PGAM1 expression in Jurkat.

ELISA: PGAM1 Antibody (2G1-A6) [H00005223-M01] - Detection limit for recombinant GST tagged PGAM1 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 2G1-A6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PGAM1 Antibody (2G1-A6) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PGAM1 (AAH11678.1, 1 a.a. ~ 254 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAYKLVLIRHGESAWNLENRFSGWYDADLSPAGHEEAKRGGQALRDAGYEFDICFTSVQKRAIRTLWTVLDAIDQMWLPVVRTWRLNERHYGGLTGLNKAETAAKHGEAQVKIWRRSYDVPPPPMEPDHPFYSNISKDRRYADLTEDQLPSCESLKDTIARALPFWNEEIVPQIKEGKRVLIAAHGNSLRGIVKHLEGLSEEAIMELNLPTGIPIVYELDKNLKPIKPMQFLGDEETVRKAMEAVAAQGKAKK
• Specificity: PGAM1 - phosphoglycerate mutase 1 (brain)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PGAM1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate for WB. It has been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PGAM1 Antibody (2G1-A6) - Azide and BSA Free

• BPG-dependent PGAM 1
• EC 3.1.3.13
• EC 5.4.2.1
• EC 5.4.2.4
• PGAMA
• PGAM-Bphosphoglycerate mutase 1
• phosphoglycerate mutase 1 (brain)
• phosphoglycerate mutase A, nonmuscle form
• Phosphoglycerate mutase isozyme B

Background

PGAM is an enzyme that catalyzes 3 reactions; the isomerization of 2-PGA to 3-PGA with 2,3-DPG as a primer, the synthesis of 2,3-DPG from 1,3-DPG with 3-PGA as a primer and the degradation of 2,3-DPG to 3-PGA. There are two different isoforms of PGAM in mammals, a muscle and brain isoform. PGAM has also been linked to non-spherocytic anemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PGAM1 Antibody (H00005223-M01)(2)

Publications using H00005223-M01

• Shen J, Wang W, Wu J et al. Comparative Proteomic Profiling of Human Bile Reveals SSP411 as a Novel Biomarker of Cholangiocarcinoma. PLoS One. 2012-10-31 [PMID: 23118872]
• Kimura A, Sakurai T, Koumura A et al. High prevalence of autoantibodies against phosphoglycerate mutase 1 in patients with autoimmune central nervous system diseases. J Neuroimmunol 2009-12-05 [PMID: 19969379]

Bioinformatics

• Gene Symbol: PGAM1
• Entrez:
  • 5223(Human)
• OMIM:
  • 172250(Human)
• Uniprot:
  • AAH11678(Human)