PGAM1 Antibody (1G4) - BSA Free

Western Blot: PGAM1 Antibody (1G4) [NBP2-59418] - Lane 1: 293T cell lysates, Lane 2: Jurkat cell lysates, Lane 3: Raji cell lysates, Lane 4: A431 cell lysates, Lane 5: HeLa cell lysates

Immunocytochemistry/Immunofluorescence: PGAM1 Antibody (1G4) [NBP2-59418] - Analysis of PGAM1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PGAM1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 1G4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PGAM1 Antibody (1G4) - BSA Free Summary

• Description: Novus Biologicals Mouse PGAM1 Antibody (1G4) - BSA Free (NBP2-59418) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human PGAM1 (1-254aa) purified from E. coli
• Isotype: IgG2a Lambda
• Clonality: Monoclonal
• Host: Mouse
• Gene: PGAM1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PGAM1 Antibody (1G4) - BSA Free

• BPG-dependent PGAM 1
• EC 3.1.3.13
• EC 5.4.2.1
• EC 5.4.2.4
• PGAMA
• PGAM-Bphosphoglycerate mutase 1
• phosphoglycerate mutase 1 (brain)
• phosphoglycerate mutase A, nonmuscle form
• Phosphoglycerate mutase isozyme B

Background

PGAM is an enzyme that catalyzes 3 reactions; the isomerization of 2-PGA to 3-PGA with 2,3-DPG as a primer, the synthesis of 2,3-DPG from 1,3-DPG with 3-PGA as a primer and the degradation of 2,3-DPG to 3-PGA. There are two different isoforms of PGAM in mammals, a muscle and brain isoform. PGAM has also been linked to non-spherocytic anemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PGAM1