PFKL Antibody (S04-7A4)

Western Blot: PFKL Antibody (S04-7A4) [NBP3-19738] - Western blot detection of PFKL in C6, 3T3 cell lysates using NBP3-19738 (1:1000 diluted). Predicted band size: 85kDa. Observed band size: 85kDa.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: S04-7A4
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

PFKL Antibody (S04-7A4) Summary

• Description: Novus Biologicals Rabbit PFKL Antibody (S04-7A4) (NBP3-19738) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human PFKL (Uniprot # P17858)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PFKL
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/1000
• Theoretical MW: 85 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for PFKL Antibody (S04-7A4)

• 6-phosphofructokinase, liver type
• DKFZp686G1648
• DKFZp686L2097
• EC 2.7.1
• EC 2.7.1.11
• FLJ30173
• FLJ40909
• human liver-type 1-phosphofructokinase, EC 2.7.1.1110Phosphohexokinase
• liver-type 1-phosphofructokinase
• PFK-B
• Phosphofructo-1-kinase isozyme B
• Phosphofructokinase 1
• phosphofructokinase, liver
• phosphohexokinase

Background

Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits. The protein encoded by this gene represents the L subunit. Alternate splicing results in two transcript variants, one of which is a candidate for nonsense-mediated decay (NMD). [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PFKL