PERP Antibody

Western Blot: PERP Antibody [NB100-2912] - (1ug/ml) staining of human liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: PERP Antibody [NB100-2912] - Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PERP Antibody Summary

• Immunogen: Peptide with sequence CLPNYEDDLLGNAK corresponding to C-Terminus according to NP_071404.2.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PERP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 20 kDa band observed in human liver lysates (calculated MW of 21.4 kDa band according to NP_071404.2).
• Control:

  Human Liver Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PERP Antibody

• dJ496H19.1
• KCP1KCP-1
• Keratinocyte-associated protein 1
• KRTCAP11110017A08Rik
• p53 apoptosis effector related to PMP22
• p53 apoptosis effector related to PMP-22
• P53-induced protein PIGPC1
• PERP, TP53 apoptosis effector
• PIGPC1RP3-496H19.1
• THWkeratinocytes associated protein 1
• Transmembrane protein THW

Background

The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in the p53 network, including Chk2, p53R2, p53AIP1, Noxa, PIDD, PID/MTA2, MTBP and PERP, were identified and their genes were cloned recently. PERP, also termed PIGPC1 and THW, is a plasma membrane protein. p53 binds to the promoter of PERP and transcriptionally activates PERP gene then the translated PERP protein mediates the p53 induced apoptosis. The expression of PERP causes cell death. PERP is a mediator of p53 induced apoptosis. PERP has sequence similarity to PMP-22/gas3 and is a new member of the PMP-22/gas3 family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PERP Antibody (NB100-2912)(1)

Publication using NB100-2912

• Ihrie RA, Marques MR, Nguyen BT et al. Perp is a p63-regulated gene essential for epithelial integrity. Cell 2005-03-25 [PMID: 15797384]

Bioinformatics

• Gene Symbol: PERP
• Entrez:
  • 64065(Human)
  • 64058(Mouse)
  • 292949(Rat)
• Uniprot:
  • NP_071404.2(Human)