Peroxiredoxin 4 Antibody (566401) [Unconjugated]

Western blot shows lysates of HEK293 human embryonic kidney cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Peroxiredoxin 4 Monoclonal Antibody (Catalog # MAB5460) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Peroxiredoxin 4 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Peroxiredoxin 4 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Peroxiredoxin 4 Monoclonal Antibody (Catalog # MAB5460) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Peroxiredoxin 4 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Mouse Anti-Human Peroxiredoxin 4 Monoclonal Antibody (Catalog # MAB5460) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clone: 566401
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Peroxiredoxin 4 Antibody (566401) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Peroxiredoxin 4
  Met1-Asn271
  Accession # Q13162
• Specificity: Detects human Peroxiredoxin 4 in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRDX4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Peroxiredoxin 4 Antibody (566401) [Unconjugated]

• Antioxidant enzyme AOE372
• AOE372
• AOE37-2PRX-4
• EC 1.11.1.15
• Peroxiredoxin 4
• Peroxiredoxin IV
• peroxiredoxin-4
• PRDX4
• Prx4
• prx-4
• prx-IV
• thioredoxin peroxidase (antioxidant enzyme)
• Thioredoxin peroxidase AO372
• Thioredoxin-dependent peroxide reductase A0372

Background

Peroxiredoxin 4 (Prx-IV; also known as PRDX4 and thioredoxin peroxidase AOE372) is a 30-32 kDa, widely expressed cytoplasmic antioxidant enzyme that belongs to the typical 2-Cys class of the TSA/ahpC family of peroxiredoxins. It exists as either a homodimer, or heterodimer with PRDX1. PRDX4 reportedly counteracts p53‑mediated generation of ROS, and regulates NF kappa B via I kappa B-alpha . Human PRDX4 is 271 amino acids (aa) in length and contains an N-terminal poly-Leu region (aa 20‑30) and a 159 thioredoxin domain (aa 79‑237). There is one catalytic cysteine at Cys124. Over amino acids 1‑271, human PRDX4 shares 89% aa identity with mouse and rat PRDX4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRDX4
• Uniprot:
  • Q13162(Human)