Peroxiredoxin 4 Antibody (566401) [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clone: 566401
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Peroxiredoxin 4 Antibody (566401) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human Peroxiredoxin 4
  Met1-Asn271
  Accession # Q13162
• Specificity: Detects human Peroxiredoxin 4 in Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Peroxiredoxin 4 Antibody (566401) [Alexa Fluor® 488]

• Antioxidant enzyme AOE372
• AOE372
• AOE37-2PRX-4
• EC 1.11.1.15
• Peroxiredoxin 4
• Peroxiredoxin IV
• peroxiredoxin-4
• PRDX4
• Prx4
• prx-4
• prx-IV
• thioredoxin peroxidase (antioxidant enzyme)
• Thioredoxin peroxidase AO372
• Thioredoxin-dependent peroxide reductase A0372

Background

Peroxiredoxin 4 (Prx-IV; also known as PRDX4 and thioredoxin peroxidase AOE372) is a 30-32 kDa, widely expressed cytoplasmic antioxidant enzyme that belongs to the typical 2-Cys class of the TSA/ahpC family of peroxiredoxins. It exists as either a homodimer, or heterodimer with PRDX1. PRDX4 reportedly counteracts p53‑mediated generation of ROS, and regulates NF kappa B via I kappa B-alpha . Human PRDX4 is 271 amino acids (aa) in length and contains an N-terminal poly-Leu region (aa 20‑30) and a 159 thioredoxin domain (aa 79‑237). There is one catalytic cysteine at Cys124. Over amino acids 1‑271, human PRDX4 shares 89% aa identity with mouse and rat PRDX4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.