Peroxiredoxin 2 Antibody (477719)

Western blot shows lysates of Raji human Burkitt's lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Peroxiredoxin 2 Monoclonal Antibody (Catalog # MAB3489) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Peroxiredoxin 2 at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 477719
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Peroxiredoxin 2 Antibody (477719) Summary

• Immunogen: E. coli-derived recombinant human Peroxiredoxin 2
  Met1-Asn198
  Accession # P32119
• Specificity: Detects human, mouse, and rat Peroxiredoxin 2 in Western blots. In Western blots, approximately 25%-40% cross-reactivity with recombinant human (rh) Peroxiredoxin 1 and 4 is observed, and less than 10% cross-reactivity with rhPeroxiredoxin 3, 5, and 6 is observed. In direct ELISAs, approximately 15%-25% cross-reactivity with rhPeroxiredoxin 1 and 4 is observed, and less than 5% cross-reactivity with rhPeroxiredoxin 3, 5, or 6 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRDX2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Peroxiredoxin 2 Antibody (477719)

• EC 1.11.1
• MGC4104
• Natural killer cell-enhancing factor B
• natural killer-enhancing factor B
• NKEF-B
• NKEFBNKEF-B
• Peroxiredoxin 2
• peroxiredoxin-2
• PRDX2
• PRPTDPX1
• PRX2
• PRXII
• TDPX1
• thiol-specific antioxidant 1
• Thiol-specific antioxidant protein
• Thioredoxin peroxidase 1
• Thioredoxin-dependent peroxide reductase 1
• torin
• TPX1
• TSA
• TSAEC 1.11.1.15

Background

Human peroxiredoxin-2 (Prx-2 or PRDX2; also Thioredoxin Peroxidase 1) is a 22 kDa antioxidant enzyme that belongs to the typical 2-Cys class of the THP/ahpC family of proteins. The precursor molecule is 198 amino acids (aa) in length, and has two catalytic cysteines, one at Cys50 and a second at Cys171. Prx-2 is an obligate homodimer. Inactive, it is apparently noncovalently associated. Upon peroxide binding to Cys50 of subunit 1, the Cys171 of subunit 2 interacts with Cys50 of subunit 1 to complete the antioxidation, generating a disulfide bond between Cys50 and Cys171. Subsequent reduction restores the subunits to the basal state. There are two additional isoforms. Isoform-b shows a deletion of aa 36‑86, while isoform c shows a substitution of 56 aa after residue 86. Human Prx-2 is 93% aa identical to mouse and rat Prx-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Peroxiredoxin 2 Antibody (MAB3489)(1)

Publication using MAB3489

• Van Brussel , Ilse, Schrijvers , Dorien M, Martinet , Wim, Pintelon , Isabel, Deschacht , Maartje, Schnorbusch , Kathy, Maes , Louis, Bosmans , Johan M, Vrints , Christia, Adriaensen , Dirk, Cos , Paul, Bult , Hidde Transcript and protein analysis reveals better survival skills of monocyte-derived dendritic cells compared to monocytes during oxidative stress. PLoS ONE, 2012-08-15;7(8):e43357. 2012-08-15 [PMID: 22916248] (ICC, Western Blot, Human)

Bioinformatics

• Gene Symbol: PRDX2
• Uniprot:
  • P32119()