Peroxiredoxin 2 Antibody (477719)

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Western blot shows lysates of Raji human Burkitt's lymphoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Mouse ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clone
477719
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Peroxiredoxin 2 Antibody (477719) Summary

Immunogen
E. coli-derived recombinant human Peroxiredoxin 2
Met1-Asn198
Accession # P32119
Specificity
Detects human, mouse, and rat Peroxiredoxin 2 in Western blots. In Western blots, approximately 25%-40% cross-reactivity with recombinant human (rh) Peroxiredoxin 1 and 4 is observed, and less than 10% cross-reactivity with rhPeroxiredoxin 3, 5, and 6 is observed. In direct ELISAs, approximately 15%-25% cross-reactivity with rhPeroxiredoxin 1 and 4 is observed, and less than 5% cross-reactivity with rhPeroxiredoxin 3, 5, or 6 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PRDX2
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publication using
MAB3489 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Peroxiredoxin 2 Antibody (477719)

  • EC 1.11.1
  • MGC4104
  • Natural killer cell-enhancing factor B
  • natural killer-enhancing factor B
  • NKEF-B
  • NKEFBNKEF-B
  • Peroxiredoxin 2
  • peroxiredoxin-2
  • PRDX2
  • PRPTDPX1
  • PRX2
  • PRXII
  • TDPX1
  • thiol-specific antioxidant 1
  • Thiol-specific antioxidant protein
  • Thioredoxin peroxidase 1
  • Thioredoxin-dependent peroxide reductase 1
  • torin
  • TPX1
  • TSA
  • TSAEC 1.11.1.15

Background

Human peroxiredoxin-2 (Prx-2 or PRDX2; also Thioredoxin Peroxidase 1) is a 22 kDa antioxidant enzyme that belongs to the typical 2-Cys class of the THP/ahpC family of proteins. The precursor molecule is 198 amino acids (aa) in length, and has two catalytic cysteines, one at Cys50 and a second at Cys171. Prx-2 is an obligate homodimer. Inactive, it is apparently noncovalently associated. Upon peroxide binding to Cys50 of subunit 1, the Cys171 of subunit 2 interacts with Cys50 of subunit 1 to complete the antioxidation, generating a disulfide bond between Cys50 and Cys171. Subsequent reduction restores the subunits to the basal state. There are two additional isoforms. Isoform-b shows a deletion of aa 36‑86, while isoform c shows a substitution of 56 aa after residue 86. Human Prx-2 is 93% aa identical to mouse and rat Prx-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Peroxiredoxin 2 Antibody (MAB3489)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Bioinformatics

Gene Symbol PRDX2
Uniprot