Peroxiredoxin 2 Antibody (477719) [Alexa Fluor® 750]

• Applications: WB
• Clone: 477719
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750

Peroxiredoxin 2 Antibody (477719) [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human Peroxiredoxin 2
  Met1-Asn198
  Accession # P32119
• Specificity: Detects human, mouse, and rat Peroxiredoxin 2 in Western blots. In Western blots, approximately 25%-40% cross-reactivity with recombinant human (rh) Peroxiredoxin 1 and 4 is observed, and less than 10% cross-reactivity with rhPeroxiredoxin 3, 5, and 6 is
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Human peroxiredoxin-2 (Prx-2 or PRDX2; also Thioredoxin Peroxidase 1) is a 22 kDa antioxidant enzyme that belongs to the typical 2-Cys class of the THP/ahpC family of proteins. The precursor molecule is 198 amino acids (aa) in length, and has two catalytic cysteines, one at Cys50 and a second at Cys171. Prx-2 is an obligate homodimer. Inactive, it is apparently noncovalently associated. Upon peroxide binding to Cys50 of subunit 1, the Cys171 of subunit 2 interacts with Cys50 of subunit 1 to complete the antioxidation, generating a disulfide bond between Cys50 and Cys171. Subsequent reduction restores the subunits to the basal state. There are two additional isoforms. Isoform-b shows a deletion of aa 36‑86, while isoform c shows a substitution of 56 aa after residue 86. Human Prx-2 is 93% aa identical to mouse and rat Prx-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.