Peroxiredoxin 1 Antibody [Unconjugated]

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, CH-1 mouse B cell lymphoma cell line, A20 mouse B cell lymphoma cell line, and L6 rat myoblast cell line . PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/Rat Peroxiredoxin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3488) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for Peroxiredoxin 1 at approximately 22 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Simple Western shows lysates of Exosome Standards (HT‑29) (NBP3-11685), Exosome Standards (PC‑3) (NBP2-49856), and Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for Peroxiredoxin 1 at approximately 28 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Peroxiredoxin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3488). This experiment was conducted under reducing conditions and using the 2-40kDa separation system.

Western blot shows lysates of U2OS human osteosarcoma cell line and Peroxiredoxin 1 knockout U2OS cell line (KO). Nitrocellulose membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/Rat Peroxiredoxin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3488) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for Peroxiredoxin 1 at approximately 22 kDa (as indicated) in the parental U2OS cell line, but is not detectable in knockout U2OS cell line. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Peroxiredoxin 1 at approximately 29 kDa (as indicated) using 2 µg/mL of Goat Anti-Human/Mouse/Rat Peroxiredoxin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3488) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Peroxiredoxin 1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Peroxiredoxin 1
  Met1-Lys199
  Accession # Q06830
• Specificity: Detects human, mouse and rat Peroxiredoxin 1 in Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human Peroxiredoxin 3 or 4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PRDX1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Simple Western 2-10 ug/mL
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Peroxiredoxin 1 Antibody [Unconjugated]

• EC 1.11.1
• EC 1.11.1.15
• Natural killer cell-enhancing factor A
• natural killer-enhancing factor A
• NKEFA
• NKEF-A
• PAG
• PAGAMSP23
• PAGB
• Peroxiredoxin 1
• peroxiredoxin-1
• PRDX1
• proliferation-associated gene A
• Proliferation-associated gene protein
• PRX1
• PRXI
• TDPX2
• Thioredoxin peroxidase 2
• Thioredoxin-dependent peroxide reductase 2

Background

Human Peroxiredoxin 1 (Prx-1 or PRDX1; also Thioredoxin Peroxidase 2) is a 22 kDa antioxidant enzyme that belongs to the typical 2-Cys class of the THP/ahpC family of proteins. The molecule is 199 amino acids (aa) in length, and has two catalytic cysteines, one at Cys52, and a second at Cys173. Prx-1 is an obligate homodimer. Inactive, it is apparently noncovalently associated. Upon peroxide binding to Cys52 of subunit 1, the Cys173 of subunit 2 interacts with Cys52 of subunit 1 to complete the antioxidation, generating a disulfide bond between Cys52 and Cys173. Subsequent reduction restores the subunits to the basal state. There are apparently two additional isoforms. One shows a premature truncation after aa 171, while the second shows a deletion of aa 21 - 121. Human Prx-1 shows 96% and 98% amino acid identity to mouse and rat Prx-1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Peroxiredoxin 1 Antibody (AF3488)(2)

Publications using AF3488

• S Jobbagy, DA Vitturi, SR Salvatore, L Turell, MF Pires, E Kansanen, C Batthyany, JR Lancaster, BA Freeman, FJ Schopfer Electrophiles modulate glutathione reductase activity via alkylation and upregulation of glutathione biosynthesis Redox Biol, 2018-11-22;21(0):101050. 2018-11-22 [PMID: 30654300] (Western Blot, Mouse)
• Radwan M, Miller I, Grunert T, Marchetti-Deschmann M, Vogl C, O'Donoghue N, Dunn MJ, Kolbe T, Allmaier G, Gemeiner M, Muller M, Strobl B The impact of tyrosine kinase 2 (Tyk2) on the proteome of murine macrophages and their response to lipopolysaccharide (LPS). Proteomics, 2008-09-01;8(17):3469-85. 2008-09-01 [PMID: 18683816] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: PRDX1
• Uniprot:
  • Q06830()