Peroxiredoxin 1 Antibody [Alexa Fluor® 647]

• Applications: WB, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 647

Peroxiredoxin 1 Antibody [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human Peroxiredoxin 1
  Met1-Lys199
  Accession # Q06830
• Specificity: Detects human, mouse and rat Peroxiredoxin 1 in Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human Peroxiredoxin 3 or 4 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Human Peroxiredoxin 1 (Prx-1 or PRDX1; also Thioredoxin Peroxidase 2) is a 22 kDa antioxidant enzyme that belongs to the typical 2-Cys class of the THP/ahpC family of proteins. The molecule is 199 amino acids (aa) in length, and has two catalytic cysteines, one at Cys52, and a second at Cys173. Prx-1 is an obligate homodimer. Inactive, it is apparently noncovalently associated. Upon peroxide binding to Cys52 of subunit 1, the Cys173 of subunit 2 interacts with Cys52 of subunit 1 to complete the antioxidation, generating a disulfide bond between Cys52 and Cys173. Subsequent reduction restores the subunits to the basal state. There are apparently two additional isoforms. One shows a premature truncation after aa 171, while the second shows a deletion of aa 21 - 121. Human Prx-1 shows 96% and 98% amino acid identity to mouse and rat Prx-1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.